猪圆环病毒2型几种ELISA抗体检测试剂盒的比较与应用  

Comparison and application of several antibody detection kits for porcine circovirus type 2

作　　者：张志慧[1] 危艳武[1] 吴洪丽[1] 黄立平[1] 李胜斌[1] 刘建波[1] 张辉[1] 王一平[1] 刘丹[1] 刘长明[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2014年第2期129-133,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：公益性行业(农业)科研专项:"重大动物疫病疫苗临床免疫评价技术研究与示范"(201203039)

摘　　要：为了比较猪圆环病毒2型（PCV2）5种ELISA抗体检测试剂盒的使用效果，本研究采用IPMA法标定不同PCV2抗体效价的阳性血清50份和阴性血清30份，对这些试剂盒的敏感性、特异性及符合率进行比较。结果显示，这5种试剂盒的敏感性为74．0％～96．0％，特异性为83．3％～96．7％，符合率为77．5％～96.3％，其中以国产阻断ELISA试剂盒和Ingenasa公司的试剂盒效果最好。此外，本研究采用国产阻断ELISA试剂盒对PCV2疫苗免疫猪血清抗体检测表明，疫苗免疫21d后抗体阳性率达50％，免疫28d抗体阳性率达100％；对PCV2人工感染猪血清抗体检测显示，病毒接种后第28d抗体阳性率为30．0％，第42d阳性率为100％；对临床送检猪血清抗体检测表明，PCV2抗体阳性率介于45.0％～76．5％。本研究通过对PCV25种抗体检测试剂盒的比较及应用显示，国产阻断ELISA试剂盒可以作为检测PCV2疫苗免疫及临床感染猪血清抗体的有效方法。To compare 5 commercial ELISA kits for detection PCV2 sera antibody, a total of 50 samples of PCV2-positive sera at different antibody titers and 30 samples of PCV2-negative sera calibrated by immunoperoxidase monolayer assay （IPMA） were used for the detections. The results showed that the sensitivity, specificity and coincidence rates were 74.0% to 96.0%, 83.3% to 96.7%, and 77.5% to 96.3% among the 5 ELISA kits, respectively. Of which a Chinese blocking ELISA kit and the kit of Ingenasa Company were the best. In addition, with the Chinese blocking ELISA kit to detect the antibody of PCV2 vaccine immunized pigs serum showed that antibody positive rate was 50% at 21 days post-vaccination （dpv） and its positive rate was 100% at 28 dpv; To detect PCV2-infected pig sera showed that antibody positive rate was 30% the at 28 days post-inoculation （dpi） and its positive rate reached 100% at 42 dpi; To detect clinical pig serum samples showed that the antibody positive rate was from 45.0% to 76.5%. In conclusion, the results suggested that the Chinese blocking ELISA kit could effectively monitor PCV2 antibody level in pigs post vaccination or natural exposure.

关 键 词：猪圆环病毒2型 抗体检测试剂盒 比较和应用 

分 类 号：S852.65[农业科学—基础兽医学]