冬小麦小RNA高通量测序及生物信息学分析  

Solexa Sequencing and Bioinformatics Analysis of Small RNA in Winter Wheat

作　　者：黄儒[1] 苍晶[1] 于晶[1] 卢宝伟[2] 刘丽杰[1,3] 王健飞[1] 郭人铭 徐琛[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]黑龙江生态工程职业学院,哈尔滨150025 [3]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006

出　　处：《植物学报》2014年第1期8-18,共11页Chinese Bulletin of Botany

基　　金：高等学校博士学科点专项科研基金(No.20112325110003);国家自然科学基金人才培养能力提高科研训练项目(No.J1210069)

摘　　要：东农冬麦1号是我国首例可在黑龙江高寒地区种植的强抗寒冬小麦(Triticum aestivum)品种。鉴于分蘖节的耐寒程度直接影响植株的安全越冬,且MicroRNAs是一类生物体内普遍存在的非编码内源小分子RNA,在植物的生长发育和适应胁迫等过程中具有重要作用,该文基于HiSeq深度测序原理,对5°C和–10°C胁迫下的东农冬麦1号分蘖节进行测序,并开展生物信息学分析。结果表明,在5°C文库中有2 229 955条特有小分子RNA序列,并检测到35条已知miRNA,属于30个miRNA家族,其表达丰度介于1–173 810之间;在–10°C文库中有3 721 449条特有小分子RNA序列,并发现29条已知miRNA,属于24个miRNA家族,其表达丰度为1–105 868。靶基因预测结果表明,5°C文库的30个miRNA家族中共预测到53个靶基因。功能分析结果显示,这些基因主要参与转录调节、新陈代谢、胁迫响应和信号转导等过程。已知小麦miRNA的类型和表达丰度在5°C和–10°C两个文库中均有较大差异。Dongnongdongmai 1 is the first cultivated winter wheat variety that can survive in Heilongjiang province. The cold tolerance of the tillering nodes directly affects the ability of overwintering. MicroRNAs （miRNAs） are a class of small non-coding endogenous RNAs with a critical role in development and environmental responses in plants. We analyzed the small RNAs of tillering nodes of Dongnongdongmai 1 under 5℃ and -10℃. Under 5℃ condition, we detected 2 229 955 unique sequences and 35 known miRNAs, which can be grouped into 30 miRNA families, with abundance ranging from 1 to 173 810. Under-10℃ condition, we detected 3 721 449 unique sequences and 29 known miRNAs, which can be grouped into 24 miRNA families, with abundance ranging from 1 to 105 868. Target gene prediction revealed 53 putative target genes from the 30 miRNAs at 5℃, some of which were involved in transcription regulation, metabolism, stress response and signal transduction. Both types and abundance of known miRNAs differed in Dongnongdongmai 1 grown at 5℃ and -10℃.

关 键 词：冬小麦 东农冬麦1号 MICRORNA 高通量测序 生物信息学 

分 类 号：S512.11[农业科学—作物学]