一种小麦叶片气孔保卫细胞观察样品的制备方法  

A Method to Improve Sample Preparation for Observing Stomata Guard Cells in Triticum aestivum

作　　者：赵林姝[1] 刘录祥[1] 古佳玉[1] 郭会君[1] 李军辉[1] 谢永盾[1] 赵世荣[1] 

机构地区：[1]中国农业科学院作物科学研究所/国家农作物航天诱变技术改良中心,北京100081

出　　处：《植物学报》2014年第1期120-126,共7页Chinese Bulletin of Botany

基　　金：国家高技术研究发展计划(No.2012AA101202);中国农业科学院中央级基本科研业务费预算增量项目(No.2013ZL007);国际原子能机构项目(RAS/5/056)

摘　　要：以小麦(Triticum aestivum)花粉植株的叶片为材料,利用整体透明技术制备小麦叶片气孔保卫细胞的观察样品,比较了4种透明剂的样品制备效果。结果表明,利用整体透明技术制备小麦叶片气孔保卫细胞观察样品,无需经过叶片撕取和刮制步骤,样品制备方法简便且高效;用甘油溶液、饱和水合三氯乙醛及水合三氯乙醛与甘油的混合液3种透明剂处理小麦叶片后,在普通显微镜下均可观察到清晰的气孔保卫细胞。Abstract We developed an efficient whole-leaf clearing method for observing stomata guard cells of anther-derived plants in Triticum aestivum. We compared the effect of 4 different clearing agents. With this method, we could obtain a sample for observing stomata guard cells in wheat without mesophyll tearing and shaving. After leaf treatment with glycerite, saturation with chloral hydrate and mixed solution of chloral hydrate and glycerin revealed clear images of stomata guard cells by microscopy. Key words anther-derived plant, ploidy identification, guard cells, Triticum aestivum, whole-leaf clearing technique

关 键 词：花粉植株 倍性鉴定 保卫细胞 小麦 整体透明技术 

分 类 号：S512.1[农业科学—作物学]