MPP+诱导的PC12细胞和星形胶质细胞共育对神经干细胞突触素和生长相关蛋白-43表达的影响  

The effect of cocultured MPP+ induced PC12 cells and astrocytes on the expression of synaptophysin and GAP-43 in neural stem cells of Wistar rat

作　　者：闫荣[1] 张尧[1] 李宇凤[1] 冯娟[1] 

机构地区：[1]中国医科大学附属盛京医院神经内科,辽宁沈阳110004

出　　处：《解剖科学进展》2014年第1期58-61,64,共5页Progress of Anatomical Sciences

基　　金：辽宁省教育厅科学技术研究(No.2009A780);辽宁省博士科研启动基金(No.20091106);沈阳市科学技术计划(F1-262-9-20)

摘　　要：目的探讨星形胶质细胞和1-甲基-4苯基-吡啶离子(MPP+)诱导的PC12细胞共育对神经干细胞(NSC)突触素和生长相关蛋白-43表达的影响。方法 PC12细胞分别经MPP+诱导不同时间点(0h、24h、48h、72h、96h)后分为两部分,一部分应用流式细胞技术检测PC12细胞凋亡率;另一部分与星形胶质细胞共育2d,然后收集各组细胞条件培养液,对神经干细胞进行诱导分化,免疫荧光检测神经干细胞突触素(SYN)和生长相关蛋白-43(GAP-43)表达。结果在MPP+作用48h时间点,PC12细胞凋亡率达高峰(P<0.05)。MPP+与PC12细胞和星形胶质细胞条件培养液共育48h可上调神经干细胞中突触素(A值=34.09±2.69)和GAP-43(A值=49.36±5.98)表达水平(P<0.05)。结论星形胶质细胞与MPP+诱导凋亡的PC12细胞共育后,促进神经干细胞突触素和GAP-43表达。Objective To study the effect of cocuhured 1-methyl-4 phenyl pyridinium（MPP ＋ ） induced PC12 cells and astrocytes on the expression of synaptophysin and GAP-43 in neural stem cells of Wistar rat. Methods PC 12 cells and MPP ＋ were cocuhured at different time（0h, 24h, 48h, 72h, 96h）as control, then the apoptosis rates of PC12 cells induced by MPP ＋ were measured by Flowcytometer. MPP ＋ and PC12 cells and astrocytes were incubated for 48 hours, then all cells-conditioned medium was collected and used to culture neural stem cells（NSCs）. The expression of synaptophysin（SYN） and growth-associated protein-43（GAP-43） in NSCs of all groups were detected by immunofluorescence. Results The apoptosis rate of PC 12 cells induced by MPP ＋ was the highest at 48h culture time, but lower at other culture hours（ P 〈 0.05）. The expression levels of synaptophysin（A value = 34.09 ＋ 2.69） and GAP-43（A value = 49.36 _＋ 5.98）in NSCs induced by astrocytes-conditioned medium cocultured for 48h were significantly upregulated compared to non astrocytes（P 〈0.05）. Conclusion Coculture of astrocytes and MPP ＋ and PC12 cells promotes the expression levels of synaptophysin and GAP-43 in NSCs.

关 键 词：星形胶质细胞 神经干细胞 突触素 生长相关蛋白-43 WISTAR大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]