云芝多糖对人CIK细胞及其杀伤功能相关分子表达的影响  

Effects of coriolus versicolor polysaccharide on human CIK cells and their cytotoxicity associated molecules

作　　者：姚萍[1,4] 陈复兴[2] 刘军权[2] 周忠海[2] 陈玲[2] 费素娟[3] 

机构地区：[1]徐州医学院,江苏徐州221004 [2]中国人民解放军第九七医院中心实验室,江苏徐州221004 [3]徐州医学院附属医院消化科,江苏徐州221002 [4]徐州市第一人民医院消化内科,江苏徐州221000

出　　处：《徐州医学院学报》2013年第12期802-805,共4页Acta Academiae Medicinae Xuzhou

基　　金：南京军区医学科技创新课题（11MA039）

摘　　要：目的 探讨云芝多糖对人CIK细胞增殖及细胞杀伤功能相关分子表达的影响.方法 体外培养CIK细胞,不同浓度云芝多糖诱导人CIK细胞24h,CCK-8比色法检测人CIK细胞增殖,流式细胞仪测定CIK细胞表达的穿孔素、颗粒酶B和CD107a的变化.结果 不同浓度的云芝多糖可促进CIK细胞增殖,促进CIK细胞穿孔素、颗粒酶B的和CD107a的表达,25 mg/L云芝多糖作用最强;但随着药物浓度的增加,CIK细胞增殖率逐渐降低,且细胞杀伤功能相关分子表达含量下降.结论 云芝多糖在低浓度下可促进CIK细胞增殖,提高CIK细胞的杀伤活性和功能.Objective To explore the effects of coriolus versicolor polysaccharide （PSK） on the proliferation of human CIK cells and the expression of cytotoxicity associated molecules. Methods PSK at different concentrations was used to induce the growth of CIK cells in vitro for 24 hours. Cell proliferation was detected by the CCK8 assay. The changes of perforin, GraB and CD107a in CIK cells were examined by flow cytometry. Results PSK could stimulate the proliferation of CIK cells and increase the expression of perforin, granzyrne B, and CD107a in CIK cells. 25 μg/mL PSK produced the strongest effect （P 〈 0.05 ）. With the rising of PSK concentrations, the growth rates of CIK cells were reduced while the content of cyotoxicity associated molecules was decreased. Conclusion Low concentrations of PSK can promote the growth of CIK cells and improve their cytotoxicity and function.

关 键 词：云芝多糖 CIK细胞 穿孔素 颗粒酶B CD107a 

分 类 号：R392.12[医药卫生—免疫学] Q26[医药卫生—基础医学]