pEGFP-N1-PEDF重组质粒的构建和鉴定  

Construction and identification of eukaryotic expression vector pEGFP-N1-PEDF

作　　者：李春明[1] 张颖鹏[1] 刘志刚[1] 谭艳平[1] 

机构地区：[1]南昌大学第二附属医院皮肤科,南昌330006

出　　处：《江西医药》2013年第12期1111-1112,共2页Jiangxi Medical Journal

基　　金：国家自然科学基金(81260238);江西省自然科学基金(20132BAB215021);江西省卫生厅资助项目(20121076)

摘　　要：目的构建pEGFP-N1-PEDF真核表达质粒,并进行鉴定。方法利用RT-PCR方法从组织中提取PEDF的基因片段,与pMD18-T载体连接,构建pMD18-T-PEDF重组质粒,酶切后与pEGFP-N1真核表达载体连接,构建pEGFP-N1-PEDF真核表达质粒,进行测序、酶切鉴定,表明质粒构建成功。结果本实验成功构建了pEGFP-N1-PEDF真核表达质粒。结论为进一步研究利用PEDF基因治疗皮肤肿瘤提供实验基础。Objective To construct an eukaryotic expression vector carrying PEDF gene. Methods PEDF gene was cloned from tissue by using RT-PCR method and was inserted into pMD18-T to construct pMD18-T-PEDF plasmid ,and to construct pEGFP-N1-EDF plasmid by using restriction enzyme.To identify the plasmid by sequencing and restriction enzyme. Results The eukaryotic expression vector pEGFP-N1-PEDF was constructed and identified. Conclusion It provides the base of apply the PEDF in treatment of skin tumor.

关 键 词：pEGFP—N1 PEDF 基因 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]