黄芩醇提液对甲型流感病毒核蛋白基因表达的影响  

Study of ethanol extracted Scutellaria on influenza A virus nucleoprotein

作　　者：李平[1] 张清[1] 孙坚[1] 

机构地区：[1]南昌大学第四附属医院呼吸与危重症医学科,南昌330003

出　　处：《江西医药》2013年第12期1128-1130,共3页Jiangxi Medical Journal

摘　　要：目的探讨黄芩醇提液对甲型流感病毒核蛋白(NP)的作用。方法本实验设HeLa细胞组、pcDNA3.1(+)/empty组、pcDNA3.1(+)/NP组、TFSB组,其中pcDNA3.1(+)/empty组、pcDNA3.1(+)/NP组分别通过瞬时转染将重组质粒pcDNA3.1(+)/empty、pcDNA3.1(+)/NP转染到HeLa细胞中;黄芩醇提液组在将重组质粒pcDNA3.1(+)/NP转染到HeLa细胞的同时使用黄芩醇提液进行药物干预。结果与真核重组质粒pcDNA3.1(+)/NP组比较,黄芩醇提液组NP基因起始拷贝数具有统计学意义,P<0.05。结论黄芩醇提液能够下调甲型流感病毒NP基因的表达。Objective To investigate the role of ethanol extracted Scutellaria on influenza A virus nucleoprotein and the molecular mechanism of skullcap anti-influenza. Methods The experimental set up HeLa cell group,pcDNA3.1 （＋）/empty group、pcDNA3.1（＋）/NP group,ethanol extracted Scutellaria group. pcDNA3.1（＋）/empty group and pcDNA3.1（＋）/NP group transient trans-fected pcDNA3.1 （＋）/empty and pcDNA3.1 （＋）/NP into HeLa cells,respectively; the ethanol extracted Scutellaria group not only transfected pcDNA3.1 （＋）/NP into HeLa cells,also interfere with ethanol extracted Scutellaria. Fluorescent quantitative RT-PCR measured the amount of nucleic acids. Results To compared with eukaryotic recombinant plasmid pcDNA3.1 （＋）/NP group,the start copy number of NP gene have statistically significant of ethanol extracted Scutellaria group.（P〈0.05）. Conclusion Ethanol extracted Scutellaria could down-regulate the expression of NP gene.

关 键 词：黄芩醇提液 核蛋白 抗流感病毒药 荧光定量RT-PCR 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]