广西莪术水提物对大鼠肝脏胞浆液抗氧化酶和微粒体药物代谢酶的影响  

Effect of aqueous extract of Curcuma kwangsiensis rhizoma on liver antioxidant enzymes in cytosols and drug metabolizing enzymes in microsomes in liver of rats

作　　者：杨秀芬[1] 石卫州[2] 程允相[1] 樊星花[1] 

机构地区：[1]广西中医药大学药学院药理学教研室,广西南宁530001 [2]广西中医药大学第一附属医院药剂科,广西南宁530023

出　　处：《中成药》2014年第2期221-224,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81060353);"教育部新世纪优秀人才支持计划人选"资助项目(教技函2010-14号;NCET-10-0093);广西自然科学基金项目(2011GXNSFF018006)

摘　　要：目的研究广西莪术水提物对大鼠肝脏胞浆液和微粒体内抗氧化酶和药物代谢酶的影响。方法用差速离心法制备肝脏胞浆液及微粒体,测定抗氧化酶和药物代谢酶NADPH-细胞色素P-450还原酶、CYP3A、CYP2E1和谷胱甘肽-S-转移酶(GST)和葡萄糖醛酸-转移酶(UGT)的活性。结果在肝胞浆液,与空白组比较,广西莪术各剂量组对超氧化物歧化酶(SOD)和谷胱甘肽还原酶(GR)水平的影响没有明显差异(P>0.05);中剂量显著增高过氧化氢酶(CAT)活性(P<0.01);高剂量明显增高GSH-PX活性(P<0.05)。广西莪术各剂量组对CYP2E1和UGT的活性均没有显著的影响(P>0.05);均明显增高GST活性(P<0.05);低剂量显著降低NADPH-细胞色素P-450还原酶活性(P<0.05),各剂量组明显降低CYP3A活性(P<0.05);苯巴比妥钠组明显增高CYP3A、GST和UGT的活性(P<0.05)。结论广西莪术可能会影响体内药物代谢,合并用药时,应考虑潜在的药物间相互作用。ABSTRACT ： AIM To study the effect of aqueous extract of Curcuma kwangsiensis rhizom （ECKR） on liver an- tioxidant enzymes in cytosols and drug metabolic enzymes in microsomes in liver of rats. MEI-ITODS Cytosols and microsomes were isolated from liver and prepared by differential centrifugation using the standard procedure. The activities of antioxidant enzymes and drug metabolic enzymes were determined by UV-Vis spectrophotometer. RESULTS In cytosols, all dosages of ECKR showed no effect on SOD and GR （P 〉 0.05） compored to the con- trol group. Medium dosage （2.43 g/kg） significantly increased the activities of catalase （CAT） （P 〈 0.05 ）, and the highest dose sharply increased the activities of GSH-PX （P 〈 0. 05 ）. In microsomes, compared with the control group, all dosages of ECKR had no effect on the activities of CYP2E1, UGT＇s activities, but all significantly increased the activities of GST （P 〈 0.05）. Low dosage （ECKR 0. 81 g/kg） decreased the activities of NADPH cytochrome P450 reductase （P 〈0. 05）. All of ECKR groups significantly reduced the activities of CYP3A （P 〈 0. 05）. The positive control group （phenobabital） induced the activity of CYP3A, GST and UGT obviously （P 〈 0. 05）. CONCLUSION ECKR may affect drug metabolism in vivo. There fore, drug-drug interactions should be considered when ECRR is used with other drugs.

关 键 词：广西莪术 抗氧化酶 药物代谢酶 

分 类 号：R285.5[医药卫生—中药学]