UHPLC-MS/MS同时测定大鼠血浆中6种木脂素类成分及其药代动力学研究  

Simultaneous determination of six lignans compounds in rat plasma and pharmacokinetics by UHPLC-MS /MS

作　　者：王青[1] 王莹 宋小妹[3] 张伟东 王鹏远 顾宜 王晓娟 

机构地区：[1]陕西国际商贸学院,陕西咸阳712046 [2]第四军医大学口腔医院药剂科,陕西西安710032 [3]陕西中医学院药学院,陕西咸阳712046

出　　处：《中成药》2014年第2期266-271,共6页Chinese Traditional Patent Medicine

摘　　要：目的建立利用UHPLC—MS／MS法同时测定大鼠血浆中GomisinD、GomisinE、五味子醇甲、五味子甲素、GomisinR和五味子乙素6种木脂素的分析方法，并研究大鼠灌胃给予五味子提取物后的药代动力学特征。方法采用AgilentPoroshell120EC—C18色谱柱，流动相为乙腈_o．1％甲酸水溶液梯度洗脱，采用电喷雾电离源（ESI），扫描方式为多反应离子监测（MRM）。结果大鼠血浆中6种木脂素成分的校正曲线线性关系良好，日内、日间精密度和准确度符合要求，提取回收率75．76％-88．82％。结论该方法简便、快速、灵敏、稳定，适用于五味子提取物的体内分析，为其进一步研究奠定基础。ABSTRACT： AIM To establish a UHPLC-MS/MS analytical method for simultaneous determination of six lig- nans compounds of Gomisin D, Gomisin E, schizandrol A, schizandrin A, Gomisin R and schizandrin B in rat plasma and to study the pharmacokinetic characteristics after oral administration of Schisandra Chiueusis Fructus. METHODS Chromatographic separation was achieved on an Agilent Poroshell 120 EC-Clscolumn, with gradient elution using a mobile phase that consisted of acetonitrile-0. 1% formic acid in water. Detetion was performed by a triple-quadrupole tandem mass spectrometer in multiple reaction monitoring （MRM） mode using the electrospray i- onization technique. RESULTS The calibration curve of six lignans compounds showed a good linear relation- ship, the intra-and inter-day precisions and accuracy met the requirements and the extraction recovery ranged be- tween 75.76% -88.82%. CONCLUSION This established method is simple, rapid, sensitive and stable and applicable to the analysis of Schisandra Chinensis Fructus for its further study.

关 键 词：UHPLC—MS MS 大鼠血浆 五味子 木脂素 药代动力学 

分 类 号：R284.1[医药卫生—中药学]