山菊降压片中生决明子代替炒决明子有效成分变化  

Content changes of stir-frying Cassiae Semen replacement with raw one in Shanju Jiangya Tablets

作　　者：张杰[1] 张振秋[1] 米宝丽[1] 曲园 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中成药》2014年第2期348-352,共5页Chinese Traditional Patent Medicine

基　　金：国家中医药行业科研专项(20110700703)

摘　　要：目的用高效液相色谱法研究山菊降压片(山楂、菊花、盐泽泻、夏枯草、小蓟、炒决明子)中生决明子代替炒决明子后绿原酸、木犀草苷、橙黄决明素、黄决明素、决明素、大黄素、大黄酚、大黄素甲醚等8个成分含有量的变化。方法采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),体积流量1.0 mL/min,柱温为30℃。流动相Ⅰ为乙腈-0.1%磷酸溶液,梯度洗脱,检测波长为348 nm,测定绿原酸、木犀草苷的量。流动相Ⅱ为乙腈-0.1%磷酸溶液,梯度洗脱,检测波长为284 nm,测定橙黄决明素、黄决明素、决明素、大黄素、大黄酚、大黄素甲醚的量。结果本方法所测定的8个成分的进样量与峰面积呈良好的线性关系,山菊降压片中生决明子代替炒决明子后,上述8个成分都有不同程度的降低,其中大黄酚、大黄素甲醚分别降低了2倍、2.8倍。结论山菊降压片中使用炒决明子较为合理。ABSTRACT： AIM To study content changes of chlorogenic acid, luteoloside, aurantio-obtusin, chryso-ob- tusin, obtusin, emodin, physcion, and chrysophanol in Shanju Jiangya Tablets when fried Cassiae Semen was re- placed by raw Cassiae Semen by HPLC. METHODS The samples were separated on an Agilent ZORBAX SB-Cls column （4. 6 mm × 250 mm, 5 ~m） column at a flow rate of 1.0 mL/min and the column temperature was at room temperature. The mobile phase I was acetonitrile-0. 1% phosphoric acid aqueous for determination of chloro- genic acid and luteoloside with gradient elution, and detection wavelength was 348 nm. The mobile phase I1 was acetonitrile-0. 1% phosphoric acid aqueous for determination of aurantio-obtusin, chryso-obtusin, obtusin, emod- in, physcion, and chrysophanol with gradient elution, and detection wavelength was 284 nm. RESULTS Eight determined constituents had a good linear relationship between content of each constituent and peak area. Eight constituents were degraded to some degrees when fried Cassiae Semen was replaced by raw Cassiae Semen. Chry- sophanol and physcion were reduced by 2 fold, respectively. CONCLUSION Using fried Cassiae Semen in Shanju Jiangya Tablets is more reasonable.

关 键 词：山菊降压片 决明子 生熟互换 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]