2型糖尿病患者PPARγ2基因Pro12Ala多态性的频率分布特点  

The frequency of the Pro12Ala single nucleotide polymorphism in peroxidase proliferation of activated receptor-γ2 gene with type 2 diabetes mellitus

作　　者：郝利霞[1,2] 毕力夫 苏秀兰[2] 

机构地区：[1]内蒙古医科大学附属医院康复科,呼和浩特010050 [2]内蒙古医科大学临床医学研究中心,呼和浩特010050 [3]内蒙古自治区卫生厅

出　　处：《中华临床医师杂志（电子版）》2013年第21期27-29,共3页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的：探讨内蒙古地区汉族2型糖尿病患者过氧化物酶增殖激活受体γ2（PPARγ2）基因Pro12Ala单核苷酸多态性的频率分布特点。方法选取2007年4月至2009年5月期间来我院就诊的糖尿病患者（依据WHO标准）97例，正常对照组55例。应用Real-Time PCR Taq Man探针法检测PPARγ2基因Pro12Ala基因型和等位基因。结果PPARγ2 Pro12Ala基因型及等位基因的分布频率分布符合H-W遗传平衡（χ2＝0.021，P＝0.885）。正常组和糖尿病组的基因型均以野生型纯合子PP（Pro/Pro型）为主。纯合子 PP、杂合子PA（Pro/Ala）基因型在糖尿病组中分别为84.5%、15.5%，在正常组中为89.1%、10.9%。PPARγ2 Pro12Ala基因型及等位基因的分布频率在糖尿病组和正常组之间差异无统计学意义（P＞0.05）。调整年龄、性别、BMI后，应用Logistic回归分析得出PP基因型与2型糖尿病的相关性，P＝0.734，OR＝1.237，95% CI：0.363～4.213。结论 PPARγ2基因Pro12Ala单核苷酸多态性与糖尿病发病不具有相关性。ObjectiveTo evaluate the frequency of Pro12Ala single nucleotide polymorphism in peroxidase proliferation of activated receptor-γ2 gene on type 2 diabetes mellitus in Inner Mongolia Han ethnic group.Methods Selected 152 cases of subjects according to WHO criteria for diagnosis of mellitus and according to BMI in type 2 diabetes mellitus （n=97） and 55 cases as the control. These subjects were evaluated for the Pro12Ala single nucleotide polymorphism in the PPARγ2 using Taqman probe in real-time Q-PCR.Results The distribution of genotype and allele frequency of PPARγ2 Pro12Ala in accordance with H-W genetic equilibrium （χ2=0.021,P=0.885）. There were given priority to with PP genotype between diabetes group and control. The frequency of PP and PA genotype in diabetes group were 84.5%, 15.5%, respectively, and in control were 89.1%, 10.9%, respectively. There was no statistically significant difference of Pro12Ala genotype and allele frequency distribution between diabetes group and control.The correlation between PP genotype and TypeⅡ diabetes by logistic regression analysis after adjustment for age, sex, BMI（P=0.734,OR=1.237, 95%CI： 0.363-4.213）. ConclusionThe Pro12Ala single nucleotide polymorphism of PPARγ2 was not associated with diabetes.

关 键 词：糖尿病 2型 PPARΓ 多态性 单核苷酸 聚合酶链反应 

分 类 号：R587.1[医药卫生—内分泌]