亚硝酸氮对红鳍东方鲀的毒性效应  

Effect of nitrite on Hepatic Antioxidant Enzymes and Acute Toxicity in Juvenile Takifugu rubripe

作　　者：汪家鑫 张钊[1] 胡盼[1] 姜志强[1] 王鑫[1] 杨晶晶[1] 王双耀[1] 

机构地区：[1]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《广东海洋大学学报》2013年第6期52-56,共5页Journal of Guangdong Ocean University

基　　金：国家海洋局公益性行业科研专项(2012418025);辽宁省科技厅重大项目(2011203005)

摘　　要：采用半静水生物测试法，研究亚硝酸盐氮对红鳍东方[鱼屯]（Takifugu rubripes）（95～9．8g）的急性毒性及对部分肝脏抗氧化酶活性的影响。结果表明，在急性毒性实验中，亚硝酸盐氮对红鳍东方[鱼屯]的24、48、72和96h半致死质量浓度（LC50）分别为20188、19057、17109、15925mg／L，安全质量浓度为1592mg／L；在亚急性毒性实验中，当红鳍东方纯分别在5．3、106，159、212mg／L亚硝酸盐氮中暴露4周时，其肝脏总抗氧化能力（T—AOC）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性随亚硝酸盐氮浓度的升高均被显著抑制，差异有统计学意义（P〈0.05），表明亚硝酸盐氮会对鱼体的抗氧化能力产生影响。The acute toxicity of nitrite to Takijhgu rubripes was studied by semi-static bioassay method at the conditions of pH 8.0, DO 7 mg/L, salinity 32 and water temperature 24℃. The results showed that the 24, 48, 72, and 96 h median lethal concentrations （LC50） of nitrite-N to Takijhgu rubripes were 201.88, 190.57, 171.09, and 159.25 mg/L respectively, as well as the safe concentration was 15.92 mg/L The subacute experimental concentrations of nitrite were set at 5.3, 10.6, 15.9, 21.2mg/L, and the exposure duration was 4 weeks, and the total antioxidant capacity （T-AOC）, superoxide dismutase （SOD） and catalase （CAT） activity in liver were used as an indicator of nitrite stress on toxicological effect. The results of sub acute experiment showed that the activities of T-AOC SOD and CAT in the liver were restrained with statistical significance （P 〈 0.05）, indicating that the nitrite had toxic effects on Takijhgu rubripes.

关 键 词：红鳍东方[鱼屯] 急性毒性 亚急性毒性 肝脏 抗氧化酶 总抗氧化能力 超氧化物歧化酶 过氧化氢酶 

分 类 号：S941.91[农业科学—水产养殖]