Studying the current properties of buffer solution through micro-fluidic channels driven with the pulse bias  

作　　者：GAO ZeYang WANG KaiGe ZHANG Chen MA HongWei WANG GuiRen BAI JinTao 

机构地区：[1]Institute of Photonics & Photo-Technology, International Scientific and Technological Cooperation Base of Photoelectric Technology & Functional Materials and Application, Northwest University [2]Mechanical Engineering Department & Biomedical Engineering Program, University of South Carolina,Columbia 29208, USA

出　　处：《Science China(Technological Sciences)》2014年第2期249-253,共5页中国科学（技术科学英文版）

基　　金：supported by the Major Research Plan of the National Natural Science Foundation of China(Grant No.91123030);the Interna-tional Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China(Grant No.2011DFA12220);the National Natural Science Foundation of China(Grant No.61378083)

摘　　要：In the research of bio-molecular chips and sensors, extra electric biases are most often employed to control and manipulate the DNA and protein molecules moving through micro/nano-fluidic channels. In order to accurately and flexibly control the bio-molecules as they move within the channels, a clear understanding of how the current changes within the buffer solution caused by an applied bias is fundamental. In this report, the current changed value of different buffer solutions, e.g., KC1, TE, and TBE was systematically studied with real-time monitoring and quantitative analysis in the situation of the buffers moving through a fluidic channel with a 5 μm inner diameter, driven by biases of 50 or 100 mV. The results revealed that the relation- ship between the current changed value and the pause interval of the applied electric field is highly consistent with the Hill Equation, which is helpful for accurately detecting and manipulating single biomolecules in microfluidic sensors and biochips.

关 键 词：micro-fluidic channel transporting mechanics current changed value interval time 

分 类 号：Q503[生物学—生物化学] TH137.52[机械工程—机械制造及自动化]