构建大鼠急性心肌梗死模型并初步探讨人脐带间充质干细胞的修复作用  

Construction of rat acute myocardial infarction model and preliminary exploration of the repairing effect of human umbilical cord mesenchymal stem cells

作　　者：赵媛媛[1] 曹文明[1] 马洁[1] 孙晓仙 孙丽[1] 钱晖[1] 许文荣[1] 朱伟[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《临床检验杂志》2013年第12期919-922,共4页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(81270214)

摘　　要：目的构建大鼠急性心肌梗死(AMI)模型并初步探讨人脐带间充质干细胞(hucMSCs)对大鼠AMI损伤的修复效果。方法用小动物呼吸机维持呼吸,开胸后快速挤出大鼠心脏,结扎左冠状动脉前降支,并结合双层荷包缝合法构建模型。经心电图,超声心动图和TTC染色多方面验证。尾静脉注射hucMSCs,观察大鼠AMI损伤修复效果。结果成功构建了大鼠AMI模型,建模后成活率高(79.2%)。与模型+PBS组左心室收缩期容积[LV Vol;s(μL):164.73±15.54]和左心室收缩期内径[LVID;s(mm):5.77±0.23]相比,模型+MSCs组[LV Vol;s(μL):116.08±9.06]和[LVID;s(mm):4.96±0.17]明显降低,F分别为75.10和107.96,P均<0.01。与模型+PBS组左心室射血分数[LVEF(%):45.55±3.71]和左心室收缩分数[LVFS(%):23.56±2.38]相比,模型+MSCs组[LVEF(%):53.99±2.32]和[LVFS(%):28.76±1.52]明显增高,F分别为65.80和62.94,P均<0.05。HE染色结果表明,模型+MSCs组细胞损伤程度明显轻于模型+PBS组。结论成功构建大鼠AMI模型并初步证明hucMSCs可以促进大鼠AMI损伤修复,为进一步研究其机制奠定了基础。Objective：To construct rat acute myocardial infarction （AMI） model and explore the repairing effect of human umbilical cord mesenchymal stem cells （hucMSCs） on the damage of AMI. Methods：In cooperation with the small animal ventilator, the rat AMI model was established by opening anocelia, extruding heart, ligating the left anterior descending coronary artery quickly and accurately, and finally closing chest combined with tightening the double purse. Next, the model was verified by using electrocardiogram, echocardiogram and TTC staining. Thereafter, hucMSCs were injected into the model rat by vena caudalis to observe their repairing effect on the damage of AMI. Results：The rat AMI models with high survival rate （79.2%） were constructed successfully. In comparison with the PBS group, the left ventricular systolic volume [LV Vol; s （μL）： 116.08 ± 9.06 vs 164.73±15.54] and left ventricular systolic diameter [LVID; s （mm）： 4.96±0.17 vs 5.77±0.23] in the MSCs group were significantly reduced （ F LV Vol; s=75.10, FLVID; s=107.96; P〈 0.01）. Meanwhile, the left ventricular ejection fraction [LVEF（%）： 53.99±2.32] and left ventricular systolic fraction [LVFS（%）： 28.76±1.52] in the MSCs group were significantly higher than that in the PBS group [LVEF（%）： 45.55±3.71; LVFS（%）： 23.56±2.38] （FLVEF=65.80, FLVFS=62.94; P〈0.05）. HE staining results of the myocardial tissue showed that the extent of cell damage in the MSCs group was obviously weaker than that in the PBS group. Conclusion：The rat AMI model was successfully constructed, and hucMSCs may promote the repair of the rat AMI damage, which lays a foundation for further investigating their mechanism of action.

关 键 词：急性心肌梗死 动物模型 人脐带间充质干细胞 

分 类 号：R541[医药卫生—心血管疾病]