Genetic Diversity of Intragenomic Rearrangement of Porcine Circovirus Type 2 in vitro and in vivo  

作　　者：Libin WEN Fengzhi WANG Bin LI Yang YU Zhengyu YU Aihua MAO Jianping XIE Kong-wang HE 

机构地区：[1]Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products

出　　处：《Agricultural Science & Technology》2013年第12期1719-1722,共4页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31272574,30972184);Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province[CX(11)2060]

摘　　要：We characterized the genome sequences of defective-interfering(DI) particle DNA of porcine circovirus type 2(PCV2) by sequencing and bioinformatics analyses. DI particles were both generated by serial passage of PCV2 in PK15 cells and obtained from sera of pigs with postweaning multisystemic wasting syndrome(PMWS). These subviral isolates ranged from 358 nt to 1 125 nt genomes. Investigating the complexity and diversity of PCV2 DI in vivo and in vitro can help elucidating the evolutionary history of PCV2.We characterized the genome sequences of defective-interfering（DI） particle DNA of porcine circovirus type 2（PCV2） by sequencing and bioinformatics analyses. DI particles were both generated by serial passage of PCV2 in PK15 cells and obtained from sera of pigs with postweaning multisystemic wasting syndrome（PMWS）. These subviral isolates ranged from 358 nt to 1 125 nt genomes. Investigating the complexity and diversity of PCV2 DI in vivo and in vitro can help elucidating the evolutionary history of PCV2.

关 键 词：Porcine circovirus type 2 Rearrangement In vivo In vitro 

分 类 号：S852.65[农业科学—基础兽医学]