慢性心衰大鼠心肌细胞内异常钙诱导钙释放的研究  

Abnormal intracellular calcium induced calcium release in chronic heart failure in rats

作　　者：胡淑婷[1] 白洁[1] 刘红梅[2] 

机构地区：[1]宁夏医科大学基础医学院生理学系,宁夏银川750004 [2]宁夏医科大学基础医学院实验中心,宁夏银川750004

出　　处：《宁夏医学杂志》2013年第12期1131-1133,I0003,共4页Ningxia Medical Journal

基　　金：国家自然科学基金资助项目(81260051);宁夏自然科学基金资助项目(NZ12191)

摘　　要：目的研究慢性心衰大鼠心肌细胞内异常的钙诱导钙释放。方法将实验动物分为手术组和假手术组,运用全细胞膜片钳、激光扫描共聚焦显微镜、蛋白印迹(Western-blot)等方法研究胞浆内钙诱导钙瞬变及钙调控相关蛋白L-型钙通道(LTCC)表达的变化。结果慢性心衰大鼠心肌细胞肌浆网内的钙瞬变(ΔF/F0=12.72±1.13)显著低于对照组大鼠心肌细胞肌浆网内的钙瞬变(ΔF/F0=28.87±2.02,P<0.01);慢性心衰大鼠心肌细胞内LTCC的表达较对照组下调。结论 LTCC表达下调所致心肌兴奋-收缩耦联的异常是导致心衰的原因之一。Objective To study abnormal intracellular calcium induced calcium release in chronic heart failure（ HF） in rats. Methods Adult male SD rats were randomized to receive either myocardial infarction（ MI） or a sham procedure. The cytosolic Ca2 ＋ induced Ca2 ＋transients were investigated and the expression of L- type Ca2 ＋channel（ LTCC） was assessed by using Patch- clamp,laser scanning confocal microscope and Western- blot. Results The cytosolic calcium transients were markedly reduced in amplitude in HF myocytes（ ΔF / F 0 = 12. 72 ± 1. 13） compared with control myocytes（ ΔF / F 0 = 28. 87 ± 2. 02,P 0. 05）,and the expression of LTCC was significantly reduced in HF rat. Conclusion Defective excitation- contraction coupling（ due to down- regulation of LTCC） could contribute to HF.

关 键 词：心力衰竭 肌浆网 L-型钙通道 钙诱导钙释放 兴奋-收缩耦联 

分 类 号：R541.6[医药卫生—心血管疾病]