口服葛根的肠代谢特征分析  

The characteristic analysis on intestinal metabolism of Pueraria lobata （Willd.）Ohwi

作　　者：李和伟[1] 赵彤[1] 董玲[1] 王刚 董红环[1] 乔延江[1] 

机构地区：[1]北京中医药大学中药学院,100102 [2]中财瀚熙(北京)生物科技发展有限公司,100055

出　　处：《国际中医中药杂志》2014年第2期131-134,共4页International Journal of Traditional Chinese Medicine

摘　　要：目的 利用中药多成分吸收代谢评价方法,考察其对同植物不同药用部位多成分吸收及其吸收代谢差异的适用性.方法 利用大鼠肠吸收代谢实验方法,对葛根素、葛根水提液、葛花水提液进行体外温孵代谢实验,并运用高效液相色谱法进行分析.结果 葛根素单一成分代谢与葛根水提液、葛花水提液多成分共同代谢的对比结果显示,葛根水提液中葛根素代谢率低于葛根素对照品溶液,酶平均浓度可能是造成此差异的原因.此外,研究发现相同植物不同药用部位的葛根与葛花的化学成分指纹图谱存在很大差异,揭示二者所含成分差异较大.结论 采用S9实验进行多成分肠吸收时的并发肠壁代谢研究,可揭示多成分的肠代谢总体特征.Objective To establish a method to compare the difference of exposure component of oral taking different part ofPueraria Lobata （Willd.） Ohwi in intestine.Methods After the incubation of puerarin,extraction Radix Puerariae and Flos Puerariae with S9,the mixtures were centrifuged to get the supernatant for analysis with HPLC.Results The research showed that the metabolic rate of puerarin was higher than that of extractions because of the concentration of enzyme.The difference between the fingerprints of Radix Puerariae and Flos Puerariae Lobata indicated that there was a difference of chemical components in such two parts of Kudzuvine Root.Conclusion The profile of intestinal metabolism can be revealed in the research of gut wall metabolism in the course of intestinal absorption by S9 incubation.

关 键 词：葛根 葛花 S9 肠代谢 

分 类 号：R285.1[医药卫生—中药学]