结核分枝杆菌Ag85复合物三维结构及抗原表位预测  

Three dimensional structure and antigen epitope prediction of Ag85 complex of Mycobacterium tuberculosis

作　　者：殷月兰 谈卫军 连凯 赵丹 徐正中 陈祥 潘志明 焦新安 

机构地区：[1]扬州大学江苏省人兽共患病学重点实验室/江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2013年第4期1-5,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31101841);江苏省自然科学基金资助项目(BK2011446);江苏省农业科技支撑计划项目(BE2012367);江苏高校优势学科建设工程项目(2011-03)

摘　　要：采用生物信息学方法对结核分枝杆菌抗原85(Ag85)复合物FbpA、FbpB和FbpC蛋白进行三维结构建模、抗原表位及互作蛋白预测。结果表明:FbpA、FbpB和FbpC均由含量较多的无规则卷曲、α螺旋及少量β折叠构成,富含细胞毒性T细胞表位和B细胞表位;此外FbpA和FbpB还具有辅助T细胞表位,说明3种蛋白具有诱导细胞免疫应答和体液免疫应答的潜能。蛋白互作预测结果表明,FbpA和FbpB同时与多种早期分泌蛋白具有互作关系。FbpA、FbpB和FbpC均含有丰富的抗原表位,可以作为检测及疫苗研究的新靶点。ABSTRACT： Mycolyl transferase Ag85 complex of Mycobacterium tuberculosis consists of three homologous proteins FbpA （Ag85A）, FbpB （Ag85B） and FbpC （Ag85C）, secrected during the early stages of infection. Three dimensional structure modeling, antigens epitopes and proteins interactions of Ag85 complex were predicted. The results showed that FbpA, FbpB and FbpC were composite proteins contained low/9-sheet content （15.1 % ）, more a-helixes content （37.2 % ） and random coils content （47.7%）. Futhermore, all of them contained cytotoxic T cell epitopes and B cell epitopes. Additionnally, FbpA and FbpB contained T helper epitopes, therefore it suggested that FbpA, FbpB and FbpC had the potentiality of inducing cellular and humoral immune response. The proteins interaction prediction showed that FbpA and FbpB could interact with some secretory proteins during early stage infection of Mycobacterium tuberculosis. These re- sults indicate that FbpA, FbpB and FbpC contain abundant epitopes and they can be utilized as the target areas in diagno- sis and vaccine research.

关 键 词：结核分枝杆菌 三维结构 抗原表位 蛋白互作 

分 类 号：R52[医药卫生—内科学] S852.61[医药卫生—临床医学]