奶牛围产期血液学和脂质过氧化指标变化的研究  

Changes in hematology and lipid peroxidation of the transition cows

作　　者：杨开红[1,2] 王家晶[1] 姚路连[1] 张家铭[1] 刘学忠[1] 朱家桥[1] 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]河南农业职业学院,河南中牟451450

出　　处：《扬州大学学报（农业与生命科学版）》2013年第4期23-26,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31372495);江苏省优势学科建设工程项目(2010-10)

摘　　要：选择产前40d的奶牛40头,每隔5d从尾静脉采集全血1次,测定血液学和血清脂质过氧化指标,探讨奶牛围产期的生理特性。结果表明:白细胞(WBC)在产前5d和分娩当天(0d)明显升高(P<0.05),产后第5天基本恢复正常,中性粒细胞数(NEU)、淋巴细胞(LYM)与WBC变化趋势一致;分娩当天红细胞(RBC)、血红蛋白(HB)和红细胞比容(HCT)均轻度升高(P<0.05);血小板(PLT)在产前5d开始升高,产后均高于产前(P<0.05);血清谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性在产前呈逐渐降低的趋势,而血清过氧化氢酶(CAT)活性在产前呈升高的趋势,但分娩时均降低(P<0.05);血清丙二醛(MDA)含量在产前呈升高的趋势,而分娩后开始逐渐降低。表明奶牛围产期发生了血液学参数的改变和氧化状态失衡的生理特性。ABSTRACT： In order to study the physiological characteristics of the transition cows, forty Holstein dairy cows at 40 d before calving were selected. Whole blood was collected from the tail vein every 5 d to conduct a detection of hematology and serum lipid peroxidation. The results showed that the WBC in 5 d before calving and on the day of calving （0 d） in- creased significantly （P〈0.05）, and returned to normal level basically from 5th d after calving. The contents of NEU and LYM were in line with the variation trend of WBC. The RBC, HB and HCT were moderately elevated （P〈 0.05） on the day of calving. PLT began to rise in 5 d before calving, the level were higher before calving than after calving （P〈 0. 05）. Serum GSH-Px and SOD activities decreased gradually before calving, while the activity of CAT was elevated. But all these decreased after calving（P〈0.05）. The content of MDA in serum was evaluated before calving, and then it started to decrease gradually after calving. In conclusion, the transition cows seem to undergo change of hematological parameters and an imbalance in the oxidative status in the transition period.

关 键 词：奶牛 围产期 血液学指标 脂质过氧化 

分 类 号：S823[农业科学—畜牧学]