桃枝枯病病原鉴定  

Identification of the pathogen causing shoot blight of peach trees

作　　者：纪兆林[1] 戴慧俊[1] 金唯新 宋宏峰[3] 张慧琴[4] 金建芳 熊彩珍 徐敬友[1] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]无锡市惠山区阳山镇农业技术综合服务站,江苏无锡214155 [3]江苏省农业科学院园艺研究所,江苏南京210014 [4]浙江省农业科学院园艺研究所,浙江杭州310021 [5]嘉兴市南湖区凤桥镇农业服务中心,浙江嘉兴314007 [6]嘉兴市南湖区林业与蚕桑站,浙江嘉兴314000

出　　处：《扬州大学学报（农业与生命科学版）》2013年第4期94-98,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家现代农业产业技术体系建设专项(CARS-31-2-02);扬州大学高层次人才科研启动基金资助项目(0274983014811)

摘　　要：为明确近年来新发现的严重危害桃树的桃枝枯病病原种类,采集浙江和江苏两地的桃枝枯病病枝,对病原进行分离和鉴定。通过对真菌分离物培养性状、分生孢子器和分生孢子形态观察,两地真菌分离物均为桃拟茎点霉(Phomopsis amygdali)。ITS1-5.8SrDNA-ITS2序列分析表明两地真菌分离物为同一种病原菌,与GenBank中桃拟茎点霉序列具有99%的相似性,其ITS序列已在GenBank中登录(登录号分别为JX081273和JX182974)。离体和活体致病性测定结果表明,分离菌株均能使桃健康新梢产生枝枯病症状,从接种发病部位再次分离到病原菌,完成了柯赫氏法则(Koch’postulates)验证。结合其形态特征、ITS序列和致病性测定结果,浙江和江苏桃枝枯病病原确定为桃拟茎点霉。ABSTRACT： Peach shoot blight is an increasingly serious and new disease in Zhejiang and Jiangsu province peach growing areas in recent years. The pathogen was isolated from peach twigs with constriction canker from peach orchards in Jiax- ing, Zhejiang province and Wuxi, Jiangsu province, respectively. Examination of the strains by morphological and cultur- al characteristics showed that the fungus was Phomopsis amygdali. The identification was verified by ITS analysis of rD- NA. The sequences （GenBank accession Nos. JX081273 and JX182974） shared 99.6% identity with each other. A com- parison of these two sequences with those in GenBank showed that the sequences had the highest nucleotide similarity （99%） with P. amygdali isolate FAU1052 from peach in the southeastern United States （accession No. AF102998）. Pathogenicity tests in vivo and in vitro indicated that the isolates caused similar reddish brown around twigs as observed in the field. The pathogen was reisolated from inoculated plants, confirming Koch＇s postulates. Based on the pathogenici- ty, morphological characteristics and rDNA molecular analysis, the pathogen of peach shoot blight was finally identified as P. amygdali.

关 键 词：桃枝枯病 形态特征 致病性 ITS序列 桃拟茎点霉 

分 类 号：S662.1[农业科学—果树学] S432.44[农业科学—园艺学]