miRNA在人骨髓来源间充质干细胞软骨诱导分化过程中的表达  

miRNA Expression during Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

作　　者：高杰[1,2] 韩建伟[2] 关凯[1] 杨彤涛[2] 李放[1] 

机构地区：[1]北京军区总医院骨科,北京100700 [2]第四军医大学唐都医院骨科,陕西西安710038

出　　处：《现代生物医学进展》2013年第30期5855-5859,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81072194)

摘　　要：目的:研究miRNAs在人骨髓来源间充质干细胞软骨诱导分化过程中的表达情况。方法:以从骨髓中分离培养的MSCs及软骨诱导培养后的细胞为实验对象,利用基因芯片检测miRNAs的表达情况,由SAM分析得到MSCs较其诱导培养细胞中差异表达的miRNAs,再进行生物信息学分析。结果:①分离培养出的MSCs经软骨诱导培养21天后,已具有软骨细胞特性,经芯片检测并SAM分析,软骨诱导培养的细胞较MSCs高表达的miRNAs有6个:hsa-miR-572、hsa-miR-130b、hsa-miR-193b、hsa-miR-28、hsa-miR-152、hsa-miR-560;软骨诱导培养的细胞较MSCs低表达的miRNAs有2个:hsa-miR-424、hsa-miR-122a。②利用TargetScan预测其靶基因,并行生物信息学分析,其中hsa-miR-130b、hsa-miR-193b、hsa-miR-152及hsa-miR-424的预测靶基因中多为参与细胞分化、骨形成、软骨形成及干细胞表型相关的基因。结论:hsa-miR-130b、hsa-miR-193b、hsa-miR-152和hsa-miR-424等对人骨髓来源间充质干细胞的软骨分化起着重要调控作用。Objective： To identify miRNA expression during chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells. Methods： MSCs were isolated by density centrifugation from bone marrow, and induced to differentiate into chondroblast. miRNA expression in MSCs and chondroblast cells was tested by miRNA microarray with single-channel fluorescence chip and analyzed using SAM software. The potential targets of the miRNAs were analyzed by bioinformatics. Results： MSCs were isolated from bone marrow and induced to differentiate into chondroblast in vitro and miRNA expression of MSCs and chondroblast were investigated using microarrays. Upon differentiation, 6 miRNAs（hsa-miR-572, hsa-miR-130b, hsa-miR-193b, hsa-miR-28, hsamiR-152, and hsa-miR-560） were up-regulated and 2 miRNAs（hsa-miR-424 and hsa-miR-122a） were down-regulated. Among the potential targets, several genes have been evidenced to play a role in osteogenic or chondrogenic differentiation and stemness maintaining. Conclusion： These finding indicate that miRNAs are likely important regulators for chondrogenic differentiation of MSCs.

关 键 词：间充质干细胞 软骨分化 MICRORNA 

分 类 号：Q953[生物学—动物学] Q75