一种冬虫夏草全基因组DNA的提取方法  

A method for extracting whole genomic DNA from Ophiocordyceps sinensis

作　　者：白飞荣 李辉[1] 刘洋[1] 姚粟[1] 扎西才吉 程池[1] 

机构地区：[1]中国食品发酵工业研究院中国工业微生物菌种保藏管理中心,北京100015 [2]玉树藏族自治州三江源药业有限公司,玉树810003

出　　处：《工业微生物》2014年第1期19-21,共3页Industrial Microbiology

基　　金：青海省科技计划项目(2012-N-142;2012-N-153)

摘　　要：冬虫夏草是真菌与昆虫形成的复合生物体,本研究建立了一种可同时提取冬虫夏草真菌子座和虫体全部基因组DNA的方法。该方法稳定高效,简便易行,提取纯度高,适用于冬虫夏草多重PCR、Realtime-PCR和DNA指纹图谱等分子水平的研究。Ophiocordyceps sinensis is a composite organism formed with fungus and insects. A method for extracting the whole genomic DNA from 0. sinensis including fungus and insects with high purity was established. The method was stable and efficient. It was suitable for multiple PCR, realtime-PCR and DNA fingerprinting analysis of 0. sinensis research.

关 键 词：冬虫夏草 基因组DNA DNA提取 

分 类 号：S567.35[农业科学—中草药栽培]