一个新的arylomycin类抗生素的研究  

Studies on a new arylomycin antibiotics

作　　者：刘佳佳[1] 饶敏 戈梅 魏维[1] 钱秀萍[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海来益生物药物研发中心,上海200240

出　　处：《中国抗生素杂志》2014年第2期98-101,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学青年基金项目(81102354)

摘　　要：目的初步鉴定一个新的脂肽类抗生素,并优化发酵条件、研究其活性。方法利用UPLC-MS/MS对其结构进行初步鉴定。在发酵培养基中添加不同的前体物质再通过HPLC方法检测目标产物的产量,最终确定最优的发酵培养基条件。通过纸片法药敏试验检测其抗菌活性。结果初步鉴定出该新的脂肽类化合物C为新化合物arylomycin A6。抗菌活性显示,该化合物在同类化合物中,对藤黄八叠球菌和表皮葡萄球菌的抗菌活性最强。结论通过优化培养基配方,获得了化合物C产量大幅度提高的条件,为后续研究打下基础。Objective To preliminarily identify a new lipopeptide antibiotics, optimize its fermentation conditions and detect its bioactivity. Methods Preliminarily identify the new lipopeptide antibiotics by ultra- performance liquid chromatography coupled with tandem quadrupole and time of flight high-resolution mass spectrometry （UPLC-Q-TOF-HRMS）. Add different precursor substances to the fermentation medium, detect the target product yield by HPLC method and ultimately determine the optimal fermentation conditions. Detect the antimicrobial activity using the disc diffusion method. Result Preliminary results indicate that the new lipopeptide compound is a new arylomycin A6. The highest yield was obtained when 3g/L isoleucine was applied. Conclusion arylomycin A2, A4 and compound C exhibit antibacterial activities against Sarcina lutea and Staphylococcus epidermidis, and compound C showed the strongest antibacterial activity amongst them.

关 键 词：脂肽类抗生素 Arylomycin 抗菌活性 

分 类 号：R978[医药卫生—药品]