疣孢菌FIM090395细胞毒活性次级代谢产物的研究  

Secondary metabolites with cytotoxic activities produced by Verrucosispora sp. FIM090395

作　　者：吴云丹[1] 聂毅磊[2] 陈名洪[2] 林如[2] 谢阳[2] 方东升[2] 彭飞[2] 连云阳[2] 江红[1,2] 

机构地区：[1]福建医科大学药学院,福州350108 [2]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《中国抗生素杂志》2014年第2期102-105,共4页Chinese Journal of Antibiotics

基　　金：福建省重点项目(2012Y0077;2011R1010-2);福建省自然科学基金(2011J01094;2012J01093);国家科技重大专项(2010ZX09401-403;2012ZX09301002-003)

摘　　要：目的研究分离自海洋沉积物的疣孢菌FIM090395产生的次级代谢产物。方法采用HP-20大孔吸附树脂、正相硅胶、Sephadex LH-20葡聚糖凝胶和反相C18柱层析等方法,对菌株FIM090395次级代谢产物进行分离纯化。通过紫外光谱、质谱、核磁共振等波谱学方法对化合物进行结构鉴定。结果与结论分离得到化合物1和化合物2,结构解析确定它们分别与proximicin A和B同质,活性研究表明化合物1对肿瘤细胞株MDA-MB-231(乳腺癌细胞)、SW620(结肠癌细胞)、SMMC7721(肝癌细胞)和CNE-2(鼻咽癌细胞)具有一定的抑制作用,其IC50值分别为23.49、13.48、34.96和16.28μg/mL;化合物2对肿瘤细胞株K562(白血病细胞)具有一定的抑制作用,其IC50值为25.69μg/mL,对MDA-MB-231和CNE-2的抑制增殖作用较弱。Objective To study the metabolites of Verrucosispora FIM090395 from marine sediments. Methods The metabolites from the culture filtrate of FIM090395 were purified by macroporous resin HP-20, silica gel, sephadex LH-20 and C18 reversed phase column chromatography. The structure of the compounds were determined by UV spectra, LC-MS,^ 1H and ^13C-NMR data analyses. Results and Conclusion Two compounds（1 and 2） were isolated from the culture filtrate, they were identified as proximicin A and B, respectively. Compound 1 showed in vitro growth inhibitory activity against the tumor cell lines MDA-MB-231, SW620, SMMC7721 and CNE-2 with ICso values of 23.49, 13.48, 34.96 and 16.28μg/mL, respectively.Therefore Compound 2 showed growth inhibitory activity against K562 cells with IC50 values of 25.69μg/mL, almost no activity against MDA-MB-231 and CNE-2 cells.

关 键 词：疣孢菌 肿瘤细胞毒活性 次级代谢产物 Proximicins 

分 类 号：R979.14[医药卫生—药品]