独活水提部位化学成分的HPLC-ESI-Q-TOF-MS分析  

Analysis on Chemical Components from Water Extract of Angelicae pubescentis Radix by High Performance Liquid Chromatography-electrospray Ionization-quadrupole-time of Flight-mass Spectrometry

作　　者：孙东东[1] 徐晓芳[2] 严世海[3] 宋小妹[2] 李祥[1] 

机构地区：[1]南京中医药大学药学院,南京210046 [2]陕西中医学院药学院,咸阳712046 [3]南京中医药大学附属医院,南京210029

出　　处：《天然产物研究与开发》2014年第1期69-76,共8页Natural Product Research and Development

基　　金：江苏高校优势学科建设工程资助项目(2011ZYX6-014);江苏省2012年度普通高校研究生科研创新计划项目(CXZZ12-0626);江苏省中医药局科技项目(LZ13013)

摘　　要：本文通过HPLC-ESI-Q-TOF-MS技术对独活水提取部分中的化学成分进行定性分析,采用LC-MS/MS,C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%甲酸溶液为流动相梯度洗脱,使用ESI离子源,正离子与负离子模式下采集数据。通过正、负离子质谱信息及元素组成,分析与相关文献数据对照,共鉴定出12个化合物,为阐明独活水提部分的药效物质基础提供有力的证据。The manuscript make a qualitative analysis on chemical components in water extract of Angelicae Pubescentis Radix by HPLC-ESI-Q-TOF-MS; LC-MS/MS, C 18 （4.6 mm×250mm,5μm） column was adopted, with methanol -0. 1% formic acid as the mobile phase for gradient elution;ESI ion source was used for mass spectra, and data were collected in positive and negative mode. twelve compounds from water extract ofAngelicae Pubescentis Radix were identified by analy- zing positive and negative ion mass spectra information and element composition and comparing controls with data from relevant literature, these provide powerful therapeutic material basis for water extract of Angelicae Pubescentis Radix.

关 键 词：独活水提液 化学成分 HPLC-ESI-Q—TOF-MS 

分 类 号：R284.1[医药卫生—中药学]