耐氧突变株对中药射干中鸢尾黄素的生物转化  

Biotransformation of Tectorigenin Extracted from Belamcanda chinensis by an Oxygen-tolerant Bacterial Mutant

作　　者：郭常亮[1] 王秀伶[1] 刘明月[1] 陈静[1] 

机构地区：[1]河北农业大学生命科学学院,保定071000

出　　处：《天然产物研究与开发》2014年第1期81-86,共6页Natural Product Research and Development

基　　金：国家自然科学基金项目(31170058);河北省百名优秀创新人才支持计划资助项目(CPRC027)

摘　　要：中药射干的甲醇提取物直接酸水解即得底物鸢尾黄素。通过本研究发现，耐氧突变株Sharpeasp．strainAeroto．Niu-O16在有氧条件下能将底物鸢尾黄素（tectorigenin）进行转化。根据产物的质谱及核磁共振氢谱和碳谱等测定结果，将菌株转化鸢尾黄索所生成的产物鉴定为二氢鸢尾黄素（dihydrotectorigenin）。耐氧突变株Aer-oto．Niu．016对底物鸢尾黄素粗品的最佳转化时间为36—48h，对底物鸢尾黄素粗品的最大转化浓度为0．8mmol／L，平均转化率为41．2％；当向培养基中添加0．15％（m／v）的￡．半胱氨酸时，底物鸢尾黄素的平均转化率可由原来的41．2％提高到50．7％。利用微生物转化法合成二氢鸢尾黄素尚属国内外首次报道，本研究为日后生物合成二氢鸢尾黄素提供了可能。The substrate teetorigenin was prepared by acid hydrolysis of the crude extract of Belamcanda chinensis. In this study,it was found that oxygen-tolerant bacterial mutant Aeroto-Niu-O16 was able to transform tectorigenin in the pres- ence of atmospheric oxygen. The metabolite of tectorigenin generated by strain Aeroto-Niu-O16 was identified as dihydro- tectorigenin based on the MS spectrometry, I H and 13C nuclear magnetic resonance （NMR） analyses. The optimal bio- converting period was 36 ~ 48 h. The maximal concentration of tectorigenin that strain Aeroto-Niu-O16 was able to trans- form effectively was 0.8 mmol/L and the average bioconverting rate was 41.2%. Furthermore,when 0. 15% （m/v） of L-cysteine was added in the cultural medium, the average bioconverting rate of the substrate tectorigenin was increased from 41.2% to 50.7%. We here showed for the first time that tectorigenin was able to be converted to dihydretectorige- nin through microbial biotransformation method. Our study provided the possibility of biosynthesizlng dihydrotectorigenin in the future.

关 键 词：射干 鸢尾黄素 二氢鸢尾黄素 耐氧细菌 微生物转化 

分 类 号：R284.2[医药卫生—中药学]