虫草发酵多糖的提取,结构分析及生物活性研究  

Extraction,Structural Identification and Bioactivities of Polysaccharide from Fermented Cordyceps

作　　者：付海田 邓超 王敏 陈敬华 

机构地区：[1]江南大学药学院,无锡214122

出　　处：《天然产物研究与开发》2014年第1期111-116,共6页Natural Product Research and Development

基　　金：教育部高等学校博士学科点专项科研基金(2011009311 0008);教育部新世纪优秀人才计划(NCET-10-0435)

摘　　要：采用热水浸提法从虫草发酵菌粉中获得水溶性虫草发酵多糖(CY),该多糖以中性糖为主要成分,分子量为9.3×103g/mol,主链由β糖苷键连接,单糖组成为木糖、甘露糖、葡萄糖和半乳糖。利用还原力、羟基自由基和DPPH体系对提取得到的多糖进行体外抗氧化活性测定,并以小鼠单核巨噬细胞RAW 264.7模型对其进行免疫增强作用的测定。结果表明,该多糖具有较好的抗氧化性,并且呈现出一定的量效关系;能促进小鼠单核巨噬细胞增殖,并增强巨噬细胞分泌NO和TNF-α、IL-1、IL-6、IL-12等细胞因子的能力,提示该多糖具有一定的免疫增强功能。The water-soluble polysaccharide （CY） was isolated from fermented Cordyceps through hot water extraction. CY,with a molecular weight of 9.3 ×10^3g/mol,was mainly composed of neutral polysaccharide. The backbone of the polysaccharide was connected with 13-glucosidic bond. The monosaccharide was consisted of xylose, mannose, glucose and galactose. The antioxidant activities of CY were investigated using reducing power,hydroxyl assay and DPPH scavenging activity assay in vitro. Additionally, the effect of CY on immunization was determined using a macrophage RAW 264.7 model. The results demonstrated that CY had antioxidant activity with a dose-dependent manner. Moreover,it induced immune enhancement effects characterized by promoting proliferation of macrophage, cellular secretion of NO and cyto- kines, such as TNF-α, IL-1, IL-6 and IL-12.

关 键 词：虫草发酵菌粉 多糖 抗氧化 免疫增强 

分 类 号：R285.5[医药卫生—中药学]