蓖麻毒素气源感染小鼠后炎性因子的定量分析  

Quantitative Analysis of Inflammatory Cytokines in Mice Air-borne Infected by Ricin

作　　者：郑关雨[1,2] 王全凯[1] 王蒙[1,2] 钱军[2,3] 刘林娜[2,3] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]军事医学科学院军事兽医研究所,吉林长春130122 [3]吉林省人兽共患病预防与控制重点实验室,吉林长春130122

出　　处：《河南科技大学学报（自然科学版）》2014年第2期74-77,9,共4页Journal of Henan University of Science And Technology:Natural Science

基　　金：国家"863"计划基金项目(2012AA022006);国家自然科学基金项目(31101858)

摘　　要：蓖麻毒素会引起机体内蛋白合成终止和细胞凋亡,通过测定组织器官内细胞因子的表达情况可监测机体中毒后免疫应答反应的发生与发展。本试验以气溶胶的方式将蓖麻毒素感染给小鼠,采用荧光定量聚合酶链式反应对攻毒后小鼠的肺脏、脾脏及胸腺中的6种炎性因子进行定量分析。研究结果显示:与对照组(生理盐水组)相比,试验组(蓖麻毒素组)小鼠肺脏、脾脏、胸腺中的趋化因子(CCL2、CXCL2)、白介素2(IL-2)和γ干扰素(INF-γ)的信使RNA(mRNA)表达均显著升高;而白介素1(IL-1)的信使RNA(mRNA)在肺脏、脾脏中高表达,在胸腺中为低表达;白介素4(IL-4)的信使RNA(mRNA)在肺脏、胸腺中高表达,在脾脏中为低表达。由此可知:经毒素气源感染后的小鼠,肺组织及免疫器官组织炎性因子分泌水平发生显著改变,局部及整体免疫调节机能紊乱。Ricin poisoning can cause the termination of protein synthesis in the body and apoptosis. This paper revealed the detail of this process and monitored the development of the immune response and cytokine storm. The mice was infected by aerosolized ricin,and the chosen 6 cytokines were determined by real time quantitative polymerase chain reaction( QRT-PCR). The results show that the mRNA of CCL2,CXCL2,IL-2, INF-γ in ricin aerosol group have a higher expression than saline aerosol group in the lung,spleen and thymus. IL-1 in ricin aerosol group is expressed higher than saline aerosol group in mice lungs and spleen,while in thymus IL-1 of ricin aerosol group is expressed lower than saline aerosol group. In lung and thymus,IL-4 is higher in ricin aerosol group,but lower in spleen. It can be conclude that after infected by ricin,the lungs, spleen and thymus of mice are subjected to a ‘cytokine storm'and immune disorder in local and whole immune system.

关 键 词：蓖麻毒素 炎性因子 荧光定量聚合酶链式反应 小鼠 

分 类 号：S852.44[农业科学—基础兽医学]