白头翁水溶性多糖提取工艺的研究  

Extraction of Water-Soluble Polysaccharides from Radix Pulsatilla

作　　者：王宏军[1] 王术德[1] 马魏[1] 李建国[1] 刘锦[1] 徐子恒[1] 王丹[1] 韩晓娜[1] 岳茹凤 

机构地区：[1]辽宁医学院,辽宁锦州121001

出　　处：《现代畜牧兽医》2014年第2期1-4,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：国家自然科学基金项目(31201951);辽宁省高等学校优秀人才支持计划项目(LJQ2013087);辽宁省中小企业创新基金(辽科发﹝2012﹞33号);辽宁省大学生创新创业训练计划项目(201310160035)

摘　　要：为了探究白头翁水溶性多糖的提取工艺,本研究采用单因素试验和L9(34)正交试验,考察料液比、提取时间、提取次数和提取温度对水溶性多糖提取率的影响,优选白头翁水溶性多糖的最佳提取工艺条件。结果表明,白头翁水溶性多糖最佳提取工艺条件为:料液比为1:17,100℃提取6 h,提取6次,白头翁水溶性多糖含量为5.07%。为白头翁水溶性多糖的进一步研究奠定了基础。To optimize the extraction procedure of water-soluble polysaccharides from Radix Pul-satilla, single factor test and orthogonal test were designed to investigate the effects of sol-id-liquid ratio, extraction time and number of extraction. The results showed that the optimal ex-tracting conditions were the solid-liquid ratio of 1：17, extraction time of 6 hrs and extracting 6 times at 100 ℃ in water. Under these conditions, the extraction percentage was up to 5.07%. This study provided the basis for further research of water-soluble polysaccharides in Radix Pulsatilla.

关 键 词：白头翁 多糖 提取工艺 

分 类 号：S859.4[农业科学—临床兽医学]