5-氮胞苷介导的转基因cry1Ab的阶段复活及其对水稻的生物学效应  

5 azacytidine mediated Phase Reactivation of cry1Ab Transgene and Its Biological Effects on the Transgenic Rice

作　　者：吴刚[1] 崔海瑞[1] 舒庆尧[1] 夏英武[1] 高明尉[1] IllimarALTOSAAR 

机构地区：[1]浙江大学原子核农业科学研究所,浙江杭州310029 [2]渥太华大学生物化学系

出　　处：《中国水稻科学》2001年第1期63-66,共4页Chinese Journal of Rice Science

基　　金：美国洛克菲勒基金资助项目

摘　　要：用不同浓度的 5 -氮胞苷溶液配合不同时间处理了发生在转录水平的转基因 cry1Ab沉默水稻。结果表明 ,5 -氮胞苷处理能使 8%～ 30 %转基因水稻植株的转基因 cry1Ab恢复表达活性 ,复活转基因表达的 Cry1Ab蛋白含量最高可达0 .147% ,45 mg/L 5 -氮胞苷处理 1d可使转基因 cry1Ab的复活率及 Cry1Ab蛋白含量达最高。同时 ,5 -氮胞苷处理使转基因水稻植株出现矮化 ,分蘖数、单株粒重下降 ,穗长减小及开花期延迟等发育异常现象。 5Different concentrations of 5 azacytidine combining with different treatment time were employed to treat the transgenic rice with a transcriptionally silenced cry1Ab transgene. The results indicated that 5 azacytidine could reactivate 8%-30% of the silenced transgenic rice plants and the expression of the reactivated cry1Ab transgene could reach as high as 0.147% of the total soluble protein. Treatment with low concentration of 5 azacytidine could lead to the highest reactivation ratio and the expression of the cry1Ab transgene. Meanwhile, treatment with azacytidine could result in dwarfism, reduction in tiller numbers, grain weight per plant, panicle length and the delaying in flowering. The study showed that 5 azacytidine could be used as a potential mutagen in overcoming of transgene silencing and creation of the transgenic plants with elite agronomic traits.

关 键 词：5-氮胞苷 转基因 基因沉默 复活 生物效应 水稻 

分 类 号：Q943[生物学—植物学] [农业科学—作物学] S511