药用裸花紫珠微繁技术增殖培养基筛选研究  

Screening Study on Proliferation Medium of Micropropagation Technique for Callicarpa nudiflora

作　　者：潘梅[1] 符瑞侃 黄赛[1] 王景飞[1] 吕德任 戚华莎 

机构地区：[1]海南省农业科学院园林花卉研究所,海南海口571100

出　　处：《北方园艺》2014年第4期96-99,共4页Northern Horticulture

基　　金：海南省科学事业费资助项目(FJSB.214.04.00.001.00);海南省自然科学基金资助项目(312084)

摘　　要：以裸花紫珠丛生芽为外植体,在光照时间9h/d,光照强度1 500lx,温度(26±2)℃,pH 5.8的培养条件下,研究比较了不同无机盐量MS培养基、不同植物生长调节剂种类及配比、蔗糖浓度、培养周期等因素对裸花紫珠生长状况的影响,以期筛选出裸花紫珠微繁技术最佳增殖培养基。结果表明:丛生芽继代增殖的最佳配方为:MS+6-BA 2.0mg/L+NAA 0.05mg/L+蔗糖30g/L,30d的增殖系数达到10以上,丛生芽长势好。Taking the clustered buds of Callicarpa nudiflora as the explant, the effect of inorganic salt types and concentrations of plant growth regulators, sucrose concentration.culture cycle and other factors on growth of Callicarpa nudiflora were studied under the conditions as light time 9 h/d,light intensity of 1500 lx, temperature of （26±2）℃ and pH 5. 8,in order to screen the optimum proliferation medium of rnicropropagation technique. The results showed that the best formula for clustered buds subculture proliferation was MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0.05 mg/L ＋ sucrose 30 g/L, proliferation coefficient was over 10 in 30 d and clustered buds growth were potential.

关 键 词：裸花紫珠 丛生芽 增殖培养 筛选 

分 类 号：Q943.1[生物学—植物学]