干旱胁迫对百脉根叶绿素荧光特性的影响  

Effects of Drought Stress on Chlorophy Ⅱ Fluorescence of Lotus corniculatus

作　　者：赵丽丽[1] 王普昶 陈超[1] 刘洪来[1] 马林[1] 

机构地区：[1]贵州大学动物科学学院草业科学系,贵州贵阳550025 [2]贵州省草业研究所,贵州贵阳550025

出　　处：《中国草地学报》2014年第1期18-23,共6页Chinese Journal of Grassland

基　　金：贵州大学引进人才科研项目[贵大人基合字(2010)036号];贵州省科学技术基金项目[黔科合J字(2010)2248];贵州省科技厅项目[黔科合NY(2010)3049号];贵州省县市科技局项目[黔西南科合字(2011)8号];贵州省优秀科技教育人才省长专项基金项目(黔省专合字[2011]39号)

摘　　要：以8个百脉根种质为材料,采用盆栽连续干旱法测定干旱胁迫不同时间各百脉根种质的叶绿素荧光参数,探讨百脉根对干旱胁迫的适应机制和抗旱性差异。研究结果表明,干旱胁迫对叶绿素荧光参数的影响非常显著(P<0.05)。随着干旱胁迫天数的增加,叶片PSⅡ的潜在活性、最大光化学效率、实际光量子产量和光化学猝灭系数等荧光参数均呈下降趋势,而叶片非光化学荧光猝灭系数则呈现先升高后降低(B01、B02和B12)或呈一直升高(B04、B05、B08、B03和B18)的变化趋势。干旱胁迫下,种质B04、B05和B08的光合机构受破坏轻,初步评定其抗旱能力较强;其次为B03和B18;B01、B02和B12的光合机构受破坏重,初步评定其抗旱性较差。Using eight Lotus corniculatus germplasm resourses as experimental materials, Chlorophy Ⅱ fluorescence parameters in leaves under drought stress with the method of pot culture consecutive drought were measured to explore the adaptation mechanisms of L. corniculatus to drought stress and to evaluate the differences of drought resistance of eight germplasm resourses. The results showed that the effect of drought stress on chlorophyll Ⅱ fluorescence parameters was very obvious（P〈0.05）. With the prolonging of the drought stress, the PS Ⅱ potential activity （Fv/Fo） , PS Ⅱ maximum photochemical efficiency （Fv/Fm） ,PS Ⅱ actual photochemical quantum yield（yield） and PS Ⅱ photochemical fluorescence quenching （qp） in all of germplasm recourses decreased significantly. The non-photochemical fluorescence quenching （NPQ） in leave of B01, B02, B12 increased at first, and then decreased as the drought stress time pro- longed, but the non-photochemical fluorescence quenching （NPQ） in leave of B04, B05, B08, B03, B18 increased steadily. Comprehensive analysis of effects of drought stress on chlorophyll Ⅱ fluorescence pa- rameters, it was suggested that the leaves of B04, B05, B08 suffered less injury, which showed better drought tolerance, followed by B03 and B18, the leaves of B01, B02, B12 suffered heavier injury, the drought tolerance of which were lower.

关 键 词：百脉根 种质 干旱胁迫 叶片 叶绿素荧光 

分 类 号：Q945.11[生物学—植物学]