附子甘草主要成分配伍对乌头碱致大鼠传代心肌细胞损伤的保护作用  

Protective effect of main component combinations of Fuzi( Radix Aconiti Lateralis praeparata ) and Gancao( Radix Glycyrrhizae ) on passage myocardial cells injured by aconitine in rats

作　　者：周天梅[1] 杨洁红 万海同[2] 张宇燕[2] 周惠芬[2] 

机构地区：[1]浙江中医药大学附属广兴医院,浙江310007 [2]浙江中医药大学

出　　处：《北京中医药大学学报》2014年第1期22-26,I0003,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81073139);高等学校博士学科点专项科研基金项目(No.20113322110002;No.20093322110001);浙江省中医药科技计划项目(No.2010ZA017);浙江省卫生高层次创新人才培养工程项目;浙江省中医药(中西医结合)重点学科(No.2012-XK-A06)

摘　　要：目的探讨附子甘草主要成分配伍对乌头碱致大鼠传代心肌细胞损伤的保护作用及机理。方法运用MTT法检测附子甘草主要成分配伍对心肌细胞存活率的影响,琥珀酸脱氢酶(SDH)试剂盒,Na+-K+ATP酶、Ca2+-Mg2+ATP酶试剂盒测定心肌细胞的SDH、Na+-K+ATP酶、Ca2+-Mg2+ATP酶活性。结果 120μmol/L浓度乌头碱组细胞存活率、SDH活性、Na+-K+ATP酶活性、Ca2+-Mg2+ATP酶活性均明显下降;而附甘不同配伍组合能使细胞存活率、SDH活性、Na+-K+ATP酶、Ca2+-Mg2+ATP酶活性均有不同程度恢复,具有显著性或极显著性差异(P<0.05或P<0.01)。其中存活率中以附甘3组改善最显著(P<0.01),SDH活性以附甘5组改善最明显(P<0.01),Na+-K+ATP酶活性、Ca2+-Mg2+ATP酶活性以附甘4组改善最明显(P<0.01),与异搏定组相比,部分有极显著性差异或显著性差异(P<0.01或P<0.05)。结论附子甘草主要成分配伍可能是通过对SDH、Na+-K+ATP酶、Ca2+-Mg2+ATP酶等进行能量代谢调节,实现其对心肌细胞损伤的保护,从而发挥减毒作用。Objective To investigate the protective effects and mechanism of the main component combinations of Fuzi （Radix Aconiti Lateralis praeparata） and Gancao （Radix Glycyrrhizae） on passage myocardial cells injured by aconitine in rats. Methods The influences of main component combinations of Fuzi and Gancao on survival rate of myocardial cells were detected by using methyl thiazolyl tetrazolium test （MTI＇）, and activities of suecinodehydrogenase （SDH）, Na＋-K＋ ATPase and CaE＋-Mg2＋ ATPase were determined by using kits of SDH, Na ＋-K ＋ ATPase and Ca： ＋ -Mg2 ＋ ATPase. Results In aconitine group （120 umol/L） , survival rate of myocardial cells and activities of SDH, Na＋ -K＋ ATPase and Ca2＋ -Mg2＋ ATPase decreased significantly. In all combination groups, survival rate of myocardial cellsand activities of SDH, Na ＋ -K ＋ ATPase and Ca2 ＋ -Mg2 ＋ATPase were recovered in varying degrees （ P 〈 0.05 or P 〈 0. 01 ）. The recovery of survival rate of myocardial cells was the most significant in combination group 3 （P 〈 0.01 ）, recovery of SDH activity was the most significant in combination group 5 （P 〈0.01 ）, and recoveries of Na＋-K~ ATPase and Ca2＋-Mg2＋ ATPase were the most significant in combination group 4 （P 〈 0.01 ） compared with verapamil group （P 〈 0.01 or P 〈 0.05 ）. Conclusion The main component combinations of Fuzi and Gancao can protect myocardial cells from injury and play decreasing toxicity role through regulating the energy metabolism of SDH, Na ＋ -K ＋ ATPase and Ca2＋- Mg2 ＋ ATPase.

关 键 词：附子 甘草 配伍 乌头碱 传代心肌细胞 琥珀酸脱氢酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]