大黄素对大鼠离体回肠平滑肌收缩的影响  

Influence of emodin on contraction of in vitro ileum smooth muscle in rats

作　　者：高柳[1] 胡畔[1] 韩笑[1] 蒋丽丽[1] 杨晶[1] 马会杰[1] 乔晓温 常爱荣 周明忠 

机构地区：[1]河北医科大学生理教研室,河北050017 [2]辛集市中医院儿科 [3]唐山市中建二局职工医院神经内科 [4]唐山市开平区卫生局卫生监督所

出　　处：《北京中医药大学学报》2014年第1期43-47,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：河北省科技厅课题(No.122777116;No.11246125D);河北省教育厅课题(No.2011145;No.2011154);河北省卫生厅课题(No.20110042)

摘　　要：目的观察大黄素(emodin)对大鼠离体回肠平滑肌收缩功能的影响,并探讨其作用机制。方法离体回肠标本随机分为7组(n=6):对照组,不同浓度大黄素组(1、5、10、20μmol/L),普萘洛尔(PRO)加大黄素组,格列苯脲(GLI)加大黄素组,NG-硝基-L-精氨酸甲酯(L-NAME)加大黄素组,无钙K-H液对照组及无钙K-H液大黄素组。采用颈椎离断法处死大鼠并分离其回肠,将肠段标本与张力换能器相连并置于氧饱和的K-H液中。运用BL-420E+生物信号采集处理系统,记录大鼠回肠平滑肌的收缩张力、幅度和频率。结果大黄素能使大鼠离体回肠平滑肌的收缩幅度明显下降,且呈浓度依赖性(P<0.05或P<0.01);对频率及张力无明显影响。PRO加大黄组(P<0.01)、GL1加大黄组(P<0.01)可部分阻断大黄素对回肠平滑肌的抑制作用,但L-NAME对大黄素所引起的回肠平滑肌的抑制作用无影响。氯化钙所引起的回肠平滑肌收缩可被大黄素所抑制(P<0.05)。结论大黄素能明显减弱大鼠离体回肠平滑肌的收缩幅度,对收缩张力和收缩频率无影响。这种作用可能是通过兴奋β肾上腺素能受体,兴奋ATP敏感钾通道,以及抑制细胞外钙内流实现。Objective To observe the influence of emodin on contraction of in vitro ileum smooth muscle in rats, and investigate the mechanism. Methods The samples of in vitro ileum were randomly divided into control group, 1 μumol/L emodin group, 5 μmoL/L emodin group, 10 μmoL/L emodin group, 20 μmoL/L emodin group, propranolol ＋ emodin group （ PRO ＋ emodin group ） , glibenclamide ＋ emodin group （ GLI ＋ emodin group） , L-NAME ＋ emodin group, calcium-free control group and calcium-free emodin group （each n = 6 ）. The rats were sacrificed by applying cervical dislocation for isolating the ileum. The samples of ileum segments were connected with tension transducer, which were then put into oxygen saturation K-H solution. The influences of emodin on contraction tension （TE）, amplitude （AM） and frequency （FR） of ileum smooth muscle were recorded by using BL-420 ＋ biological signal processing system. Results Emodin reduced significantly contraction AM of rat ileum smooth muscle （e 〈 0.05 or P 〈 0. 01 ） showing a concentration dependence, and had no significant influences oncontraction FR and TE. Propranolol （P 〈 0.01 ） and glibenclamide （P 〈 0.01 ） partially blocked up the inhibitory effect of emodin on ileum smooth muscle, but L-NAME had no effect. The contraction of ileum smooth muscle induced by calcium chloride could be inhibited by emodin （ P 〈 0.05 ）. Conclusion Emodin can significantly attenuate the contraction AM of rat ileum smooth muscle and has no effects on contraction TE and FR, which may be related to activating β-adrenergic receptor, exciting ATP sensitive potassium channel and inhibiting extracellular calcium influx.

关 键 词：大黄素 回肠平滑肌 Β肾上腺素能受体 ATP敏感钾通道 外钙内流 大鼠 

分 类 号：R285.5[医药卫生—中药学]