眼针对脑缺血再灌注模型大鼠脑组织BDNF、p75NTR表达影响及其脑保护作用机制  

Influences of eye acupuncture on expressions of BDNF and p75NTR and its cerebral protective effect in rats with cerebral ischemia-reperfusion injury

作　　者：马贤德[1] 王守岩[1] 张威[2] 

机构地区：[1]辽宁中医药大学教学实验中心,辽宁110032 [2]辽宁中医药大学附属第一医院

出　　处：《北京中医药大学学报》2014年第1期48-52,I0004,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2007CB512702);沈阳市科技局课题(No.F11-264-1-66)

摘　　要：目的观察眼针对脑缺血再灌注(MCAO/R)模型大鼠脑组织内脑源性神经营养因子(BDNF)及神经营养素受体p75(p75NTR)表达水平的影响,探讨眼针治疗脑缺血再灌注损伤的部分机制。方法 62只SD大鼠随机分为3组:空白对照组(11只)、假手术组(11只)、模型复制组(40只)。模型复制组大鼠采用线栓法建立脑缺血再灌注模型,将模型复制成功的大鼠(32只)随机均分为模型对照组及眼针治疗组。眼针治疗组大鼠参照人体取穴方法,取肝区、上焦区、下焦区、肾区,进行针刺干预。再灌注后2 h即开始针刺,每8 h 1次,连续针刺10次,末次干预后30 min,断髓处死大鼠,剥离缺血区域周围半暗带部位的脑组织,采用RT-PCR法和免疫组织化学法对各组大鼠脑组织内BDNF及p75NTR基因及蛋白表达水平进行检测。结果与空白对照组比较,模型对照组大鼠半暗带脑组织内BDNF及p75NTR基因和蛋白表达水平均显著上调(P<0.01,P<0.05);与模型对照组比较,眼针治疗组大鼠半暗带脑组织中BDNF基因和蛋白表达水平显著上调(P<0.01),p75NTR基因和蛋白表达水平显著下调(P<0.01,P<0.05)。结论脑缺血再灌注损伤发生后,可促进BDNF与其受体p75NTR的表达,以对抗损伤。眼针可通过上调BDNF,下调p75NTR表达水平而实现其脑保护作用。Abstract： Objective To observe the influences of eye acupuncture on expressions of brain-derived neui^otrophic factor （BDNF） and p75 neurotrophic receptor （p75NTR） and its cerebral protective effect in rats with cerebral ischemia-reperfusion injury, and discuss the therapeutic mechanism of eye acupuncture. Methods SD rats （ n = 62） were randomly divided into blank group （n = 11 ）, sham- operation group （ n = 11 ） and model copy group （ n = 40）. The model of cerebral ischemia-reperfusion was established by using suture method, and successfully modeled rats （n = 32） were divided into model group and eye acupuncture group. The eye acupuncture group was given needling in the acupoints in areas of liver, upper energizer, lower energizer and kidney according to the acupoint selection methods in human body after repeffusion for 2 h, once every 8 h for 10 times. The rats were killed 30 rain after thelast needling for peeling ischemic half dark cerebral tissue, and the gene and protein expressions of BDNF and p75NTR were detected by using real-time fluorescence quantitative polymerase chain reaction （RT- PCR） and immunohistochemistry technique. Results Compared with blank group, the gene and protein expressions of BDNF and p75NTR increased significantly in model group （ P 〈 O. 01 or P 〈 0.05 ）. Compared with model group, the gene and protein expressions of BDNF increased significantly （P 〈 0.01 ） , and gene and protein expressions of p75NTR decreased significantly （P 〈0. O1 or P 〈0.05 ） in eye acupuncture group. Conclusion The expressions of BDNF and p75NTR will increase for antagonizing brain injury after cerebral ischemia-reperfusion. Eye acupuncture can protect brain through up-regulating the expression of BDNF and down-regulating the expression of p75NTR.

关 键 词：眼针 脑缺血再灌注 脑源性神经生长因子 神经营养素受体p75 大鼠 

分 类 号：R285.5[医药卫生—中药学]