针刺舞蹈震颤控制区对帕金森病小鼠脑内黑质多巴胺神经元及BDNF表达的影响  

Influences of needling chorea-tremble controlled zone on expressions of dopaminergic neurons and BDNF in mice with Parkinson's disease

作　　者：冯婧[1] 孙红梅[1] 王媛媛[1] 许红[1] 吴海霞[1] 和欣[1] 高誉珊[1] 张淑静[1] 龚小钢 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《北京中医药大学学报》2014年第1期53-57,F0003,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：北京中医药大学自主课题资助项目(No.2011-JYBZZ-JS063)

摘　　要：目的通过观察针刺舞蹈震颤控制区对帕金森病(PD)小鼠脑内脑源性神经营养因子(BDNF)的表达,探讨针刺对多巴胺(DA)神经元的保护作用及可能机制。方法 C57BL/6雄性小鼠随机分为5组:正常组、模型组、针刺组、美多巴组、针美组。后4组首先由腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)建立PD动物模型,后3组在造模后采取不同治疗措施,存活28 d。采用爬杆法观察小鼠行为学;HE染色观察中脑黑质神经元的病理变化。免疫组织化学技术检测黑质多巴胺神经元的丢失程度;免疫印迹技术检测脑内BDNF的表达。结果 3个治疗组小鼠行为学评分均较模型组有明显增高(P<0.05)。造模后,小鼠黑质多巴胺神经元数目较正常组减少(P<0.05),其中针美组多巴胺神经元较美多巴组增多(P<0.05);模型组脑BDNF的表达较正常组明显降低(P<0.05),针美组的表达较模型组明显增加(P<0.05)。结论针刺舞蹈震颤控制区特别是再给予美多巴复合治疗可能通过增加脑BDNF的表达,减少PD小鼠黑质DA神经元的缺失,从而达到治疗PD的目的。Objective To investigate the protective effect and possible mechanism of acupuncture on dopaminergic neurons （DA neurons ） through observing the influences of needling chorea-tremble controlled zone on expression of substantia nigra brain derived neurotrophic factor （BDNF） in mice with Parkinson＇s disease （PD）. Methods Male C57BL/6 mice were randomly divided into normal group, model group, needling group, madopar group and needling ＋ madopar group. The later 4 groups were given intraperitoneal injection of MPTP for establishing PD model, and then the later 3 groups were given corresponding treatment for 28 d. The ethological changes were observed in mice by using climbing method. The pathological changes of midbrain substantia nigra dopaminergic neurons were observed after HE staining. The loss of DA neurons was detected by applying immunohistochemistry technique and expression of BDNF was detected by using Western blot method. Results The ethological scores increased significantly in needling group, madopar group and needling ＋ madopar group compared withmodel group （P 〈 0.05 ）. The count of DA neurons was higher in model group than that in normal group （ P 〈 O. 05 ）, and was higher in needling ＋ madopar group than that in madopar group （P 〈 O. 05 ）. The expression of BDNF decreased significantly in model group compared with normal group （ P 〈 O. 05 ）, and increased significantly in needling ＋ madopar group compared with madopar group （P 〈 O. 05 ）. Conclusion Needling chorea-tremble controlled zone combining madopar can treat PD through increasing the expression of BDNF and decreasing the loss of DA neurons in mice with PD.

关 键 词：针刺 小鼠 帕金森病 舞蹈震颤控制区 脑源性神经营养因子 

分 类 号：R285.5[医药卫生—中药学]