Th9细胞和白介素-9在细粒棘球蚴感染患者中的变化特点及意义  

The expression and significance of Th9 cells and interleukin-9 in patients infected with Echinococcus granulosus

作　　者：庞楠楠[1] 刘朝华[1] 张峰波[2] 朱玥洁[3] 李艳华 辛燕 马秀敏[2] 温浩 丁剑冰 

机构地区：[1]新疆医科大学第一附属医院血液病中心,乌鲁木齐830011 [2]新疆医科大学第一附属医院检验科,乌鲁木齐830011 [3]新疆医科大学第一附属医院生殖助孕中心,乌鲁木齐830011 [4]新疆包虫病基础医学重点实验室

出　　处：《免疫学杂志》2014年第1期49-52,共4页Immunological Journal

基　　金：国家自然科学基金项目(81060135,81160200,30860263)

摘　　要：目的探讨辅助性T细胞9(Th9)在肝囊型包虫病患者慢性感染中的作用。方法通过流式细胞术和实时荧光定量PCR法分别检测2010年8月至2012年12月新疆医科大学第一附属医院33例囊型包虫病患者(CE)及20例健康体检者外周血Th9细胞和特异性转录因子PU.1的表达。结果 1)CE组Th9细胞占CD4+T的比值(CD3+CD4+IL-9+/CD4+T cells)(0.91%±0.57%)高于健康对照组(0.61%±0.2%),差异具有统计学意义(P=0.042);2)CE组PU.1、IL-9 mRNA的表达水平明显高于健康对照组,差异均有统计学意义(P<0.05)。结论 CE患者体内Th9/IL-9表达上调,Th9细胞可能参与了细粒棘球蚴慢性感染的免疫应答和调控。To explore the role of the helper T cells 9 （Th9） in patients with Echinococcus granulosus infection, total of 33 patients with hepatic hydatid disease （CE group） who were treated at the First Affiliated Hospital of Xinjiang Medical University and 20 healthy control （HC group） were enrolled in this study. We employed flow cytometry to measure peripheral blood Th9 cells, and real-time fluorescent quantitative PCR to detect the expression of PU.1 （specific transcription factor of Th9 cell）. Result showed that the ratio of Th9 cells （CD3±CD4±IL- 9＋/CD4＋T cells） of the CE group [（0.91±0.57）%] was higher than that of healthy controls [（0.61±0.2）%] （P=0.042）; furthermore, in CE group, PU.1 and IL-9 mRNA expression levels were significantly higher as compared with healthy control group （P〈 0.05）. In conclusion, the expression of Th9/IL-9 are up regulated in CE patients, and Th9 cells may involved in human immune response and regulation after Echinococcus granulosus infection.

关 键 词：细粒棘球蚴 TH9 PU-1 IL-9 

分 类 号：R532.32[医药卫生—内科学]