新型布尼亚病毒抗体检测方法的建立及初步应用  

Establishment and Evaluation of Kit for Detecting Novel Bunyavirus Antibody

作　　者：陈堃[1] 何竞[1] 修冰水[1] 王国华[1] 宋晓国[1] 朱翠侠[1] 杨锡琴[1] 冯晓燕[1] 张贺秋[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《生物技术通讯》2014年第1期100-101,共2页Letters in Biotechnology

基　　金：国家"十二五"传染病防治重大专项(2013ZX10004803)

摘　　要：目的:建立新型布尼亚病毒IgG抗体ELISA检测方法。方法:用基因工程重组表达的新型布尼亚病毒NP抗原包被酶联板,建立间接ELISA法检测新型布尼亚病毒IgG抗体,并进行特异性和灵敏度评价,健康人群中检测结果计算临界值(均值+3标准差)。检测70例发热伴血小板减少综合征患者恢复血清和69份健康人血清样品。结果:在70份患者血清样品中,检测出新型布尼亚病毒IgG抗体阳性51例,阳性率为72.14%(51/70);69份健康人血清样品中,检测出1份阳性,特异性为98.6%(1/69)。结论:建立的新型布尼亚病毒IgG抗体ELISA检测方法特异性强、灵敏度高,可用于新型布尼亚病毒感染的检测及流行病学调查。Objective： To establish and evaluate an ELISA kit for detecting novel bunyavirus antibodies of fever with thrombocytopenia associated syndrome. Methods： The enzyme-linked reaction plate was coated by recombi nant NP antigens of novel bunyavirus, to establish the indirect ELISA kit for detecting novel bunyavirus antibody. The healthy individuals 69 and fever with thrombocytopenia associated syndrome 70 were detected. The sensitivity and specificity were evaluated. Results： The results showed that the specificity was 98,6% （1/69） from sera healthy individuals and the sensitivity was 72.14%（51/70） from sera fever with thrombocytopenia associated syn drome. Conclusion： The ROC analysis indicated that the indirect ELISA assay for detecting novel bunyavirus anti bodies had a better overall diagnostic performance, which provided useful tools for diagnosis and epidemiological study of fever with thrombocytopenia associated syndrome.

关 键 词：新型布尼亚病毒 IGG抗体 酶联免疫检测方法 

分 类 号：R392.1[医药卫生—免疫学]