Hippocampal gene expression in a rat model of depression after electroacupuncture at the Baihui and Yintang acupoints  

作　　者：Dongmei Duan Xiuyan Yang2 Institute of Health Maintenance Beijing University of Chinese Medicine Ya Tu Liping Chen 

机构地区：[1]Department of Traditional Chinese Medicine of South Building,Chinese PLA General Hospital [2]School of Acupuncture and Moxibustion,Beijing University of Chinese Medicine

出　　处：《Neural Regeneration Research》2014年第1期76-83,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81273847

摘　　要：Preliminary basic research and clinical findings have demonstrated that electroacupuncture ther- apy exhibits positive effects in ameliorating depression. However, most studies of the underlying mechanism are at the single gene level; there are few reports regarding the mechanism at the whole-genome level. Using a rat genomic gene-chip, we profiled hippocampal gene expression changes in rats after electroacupuncture therapy. Electroacupuncture therapy alleviated depres- sion-related manifestations in the model rats. Using gene-chip analysis, we demonstrated that electroacupuncture at Baihui （DU20） and Yintang （EX-HN3） regulates the expression of 21 genes. Real-time PCR showed that the genes Vgf, lgf2, Trnp32, Loc500373, Hifla, Folrl, Nrnb, and Rtn were upregulated or downregulated in depression and that their expression tended to nor- malize after electroacupuncture therapy. These results indicate that electroacupuncture at Baihui and Yintang modulates depression by regulating the expression of particular genes.Preliminary basic research and clinical findings have demonstrated that electroacupuncture ther- apy exhibits positive effects in ameliorating depression. However, most studies of the underlying mechanism are at the single gene level; there are few reports regarding the mechanism at the whole-genome level. Using a rat genomic gene-chip, we profiled hippocampal gene expression changes in rats after electroacupuncture therapy. Electroacupuncture therapy alleviated depres- sion-related manifestations in the model rats. Using gene-chip analysis, we demonstrated that electroacupuncture at Baihui （DU20） and Yintang （EX-HN3） regulates the expression of 21 genes. Real-time PCR showed that the genes Vgf, lgf2, Trnp32, Loc500373, Hifla, Folrl, Nrnb, and Rtn were upregulated or downregulated in depression and that their expression tended to nor- malize after electroacupuncture therapy. These results indicate that electroacupuncture at Baihui and Yintang modulates depression by regulating the expression of particular genes.

关 键 词：nerve regeneration acupuncture traditional Chinese medicine DEPRESSION gene expres-sion profiling GENE-CHIP ELECTROACUPUNCTURE Baihui （DU20） Yintang （EX-HN3） chronic stress behavior NSFC grant neural regeneration 

分 类 号：R245[医药卫生—针灸推拿学]