Acupuncture at Baihui and Dazhui reduces brain cell apoptosis in heroin readdicts  

作　　者：Xiaorong Hou Rongjun Zhang Hang Lv Xinghui Cai Guangchuan Xie Xiaoge Song 

机构地区：[1]Research Institute of Acupuncture and Meridian,Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Foundation and Technology of Acupuncture and Moxibustion (Cultivating Base) [2]Department of Human Anatomy, School of Integrated Traditional Chinese&Western Medicine, Anhui University of Traditional Chinese Medicine [3]Department of Acupuncture and Tuina,School of Acupuncture&Osteology,Anhui University of Traditional Chinese Medicine

出　　处：《Neural Regeneration Research》2014年第2期164-170,共7页中国神经再生研究（英文版）

基　　金：financially sponsored by the Foundation for Excellent Young Talents in Universities of Anhui Province in China,No.2010SQRL105;the National Natural Science Foundation of China,No.81173325

摘　　要：Acupuncture at Baihui （GV20） and Dazhui （GV14） reduces neuronal loss and attenuates ultra- structural damage in cerebral ischemic rats. However, whether acupuncture can treat addiction and prevent readdiction through changes to brain cell ultrastructure remains unknown. In this study, cell apoptosis was observed in the hippocampus and frontal lobe of heroin readdicted rats by electron microscopy. Immunohistochemical staining displayed a reduction in Bcl-2 ex- pression and an increase in Bax expression in the hippocampus and frontal lobe. After rats were given acupuncture at Baihui and Dazhui, the pathological damage in the hippocampus and frontal lobe was significantly reduced, Bcl-2 expression was upregulated and Bax expression was downregulated. Acupuncture exerted a similar effect with methadone, a commonly used drug for clinical treatment of drug addiction. Experimental findings suggest that acupuncture at Dazhui and Baihui can prevent brain cell apoptosis in heroin readdicted rats.Acupuncture at Baihui （GV20） and Dazhui （GV14） reduces neuronal loss and attenuates ultra- structural damage in cerebral ischemic rats. However, whether acupuncture can treat addiction and prevent readdiction through changes to brain cell ultrastructure remains unknown. In this study, cell apoptosis was observed in the hippocampus and frontal lobe of heroin readdicted rats by electron microscopy. Immunohistochemical staining displayed a reduction in Bcl-2 ex- pression and an increase in Bax expression in the hippocampus and frontal lobe. After rats were given acupuncture at Baihui and Dazhui, the pathological damage in the hippocampus and frontal lobe was significantly reduced, Bcl-2 expression was upregulated and Bax expression was downregulated. Acupuncture exerted a similar effect with methadone, a commonly used drug for clinical treatment of drug addiction. Experimental findings suggest that acupuncture at Dazhui and Baihui can prevent brain cell apoptosis in heroin readdicted rats.

关 键 词：nerve regeneration traditional Chinese medicine ACUPUNCTURE heroin readdiction braininjury ULTRASTRUCTURE Bcl-2 Bax apoptosis neuroprotection NSFC grant neural regeneration 

分 类 号：R245[医药卫生—针灸推拿学]