高血压患者内皮细胞来源小微粒的检测研究  

Study of Small Microparticles Derived from Endothelial Cells in Hypertension

作　　者：胡帅帅[1] 仉红刚[1] 张秋菊[1] 修瑞娟[1] 

机构地区：[1]北京协和医学院,中国医学科学院微循环研究所,国家卫计委微循环重点实验室,北京100001

出　　处：《微循环学杂志》2014年第1期3-5,F0002,I0001,共5页Chinese Journal of Microcirculation

基　　金：国家自然科学基金项目(11274046);协和青年基金项目(3332013021)

摘　　要：目的:检测高血压患者血浆中内皮细胞来源小微粒(SEMPs)水平,初步探讨其与高血压时内皮细胞损伤的关系。方法:招募高血压患者17例(高血压组),健康个体10例(健康组)。采用流式细胞术,以0.46μm的标准微球设门,检测血浆中CD62E+的SEMPs数量及阳性百分比(CD62E+%),采用ROC曲线分析SEMPs对高血压的诊断价值并判断阈值。结果:高血压患者CD62E+的SEMPs数量与健康组无显著差异(P>0.05),但其CD62E+%显著高于健康组(P<0.01)。CD62E+%的ROC曲线下面积为0.865,诊断分界点为1.15。结论:高血压患者存在SEMPs的CD62E+%异常升高,为分析高血压患者内皮细胞损伤提供了新的证据和无创性指标。Objective:To investigate of small microparticles(SEMPs)derived from endothelial cells in patients with hypertension and discuss the relativity between SEMPs and endothelial injury in hypertension.Method:17patients with hypertension(hypertension group)and 10healthy(healthy group)were enrolled.Level of small microparticles(including the quantity and positive ratio of CD62E+)derived from endothelial cells was measured by flow cytometry.The size of microparticles was defined by standard bead(0.46μm)for measurement of the level of small microparticles.SEMP value in diagnosis of hypertension was analyzed and threshold was judged by ROC curve.Results:The level of CD62E+SEMP showed no difference between patients and healthy subjects(P>0.05),but the positive ratio of it in patients was significant higher than healthy subjects(P<0.01).ROC Curve area of CD62E+% was 0.865,its Youden indexbased optimal cut-off point was 1.15.Conclusion:Thee positive ratio of CD62E+SEMP shown abnormal rise in hypertension patients,it may provide a new evidence and noninvasive marker for the analysis of hypertension on endothelial cell injury.

关 键 词：内皮细胞激活 高血压患者 内皮微粒 细胞来源 检测 心血管危险因素 生物学标记物 内皮细胞损伤 

分 类 号：R446.9[医药卫生—诊断学] R544.1[医药卫生—临床医学]