现代吸脂技术采集脂肪混合物在脂肪干细胞制备领域中的应用  

Advanced liposuction technologies and their potential application in the isolation of adipose-derived stem cells

作　　者：付寅生 张怡 

机构地区：[1]黑龙江省干细胞工程技术研究中心,黑龙江省哈尔滨市150028

出　　处：《中国组织工程研究》2014年第1期131-136,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:目前用于整容的吸脂术发展很快,但为了脂肪干细胞应用而进行的综合吸脂术研究和比较尚未见报道。目的:对比目前各种类型脂肪抽取技术的特点以及对基质血管层、脂肪细胞、脂肪干细胞的数量和存活率等指标的影响,评价出细胞活性高、适合进行脂肪干细胞制备、储存及临床科学研究的吸脂技术。方法:作者分别采用检索关键词"adipose-derived stem cells,ADSCs,liposuction,SAL,UAL,PAL,WAL"和"脂肪干细胞、脂肪抽取术、负压吸脂术、超声辅助吸脂术、震动辅助吸脂术、水动力辅助吸脂术"检索了PubMed数据库、中国知网全文期刊数据库和美国NIH临床试验数据库,排除与研究目的无关和内容重复者,保留50篇文献进行进一步分析。结果与结论:目前可应用于脂肪间充质干细胞采集的现代吸脂技术主要有负压吸脂、超声辅助吸脂、震动辅助吸脂、水动力辅助吸脂。其中负压吸脂、超声辅助吸脂技术、水动力辅助吸脂技术3类技术采集的脂肪混合物中可以分离出脂肪干细胞且具有较高的活性,可以用作自体细胞辅助移植技术和再生疗法。这4类技术虽然各有特点,有必要进一步研究这些吸脂技术获得的脂肪干细胞的活性、遗传性状稳定性、分化能力等系统性对比数据,为未来的再生医学工程提供更准确完善的支持。BACKGROUND：Currently, cosmetic liposuction has developed rapidly, but the liposuction for the isolation of adipose-derived stem cells has not been reported. OBJECTIVE：By evaluating the quantity and viability of stromal vascular fraction, adipocytes and adipose-derived stem cells obtained from different types of liposuctions, to find the advanced liposuction procedures that are most suitable for use in preparation, preservation and clinical application of adipose-derived stem cells. METHODS：Authors retrieved PubMed database, NIH clinical trial databaseand CNKI ful text database for relevant articles using the key words of“adipose-derived stem cells, ADSCs, liposuction, SAL, UAL, PAL, WAL”in English and Chinese, respectively. After eliminating the objective-independent articles and repeating reviews, 50 papers were included for further analysis. RESULTS AND CONCLUSION：Suction-assisted liposuction （SAL）, ultrasound-assisted liposuction （UAL）, power-assisted liposuction （PAL） and water-assisted liposuction （WAL） are four major types of fat-harvesting technologies that can possibly be applied for adipose-derived stem cells isolation. Accumulative evidence suggests that these advanced liposuctions can obtain high quality of adipose tissue/cells, which are good enough for autologous fat grafting. Adipose-derived stem cells that are isolated from lipoaspirate by WAL, SAL and UAL, can be used as autologous cel-assisted lipotransfer and regenerative therapy. In summary, although each of these four types of liposuction technologies has its own advantages and is feasible to be used as autologous fat grafting, systematic comparative studies for viability, chromosome stability and differentiation potentials of adipose-derived stem cells isolated using each col ecting method are necessary in order to confirm the future＆amp;nbsp;perspectives in adipose-derived stem cells isolation, banking and regenerative repair.

关 键 词：干细胞 脂肪干细胞 脂肪抽取术 负压吸脂术 超声辅助吸脂术 震动辅助吸脂术 水动力辅助吸脂术 

分 类 号：R318[医药卫生—生物医学工程]