电烙铁损伤左侧外周鼻孔区构建单侧嗅觉剥夺模型:嗅球神经的发生和转化  

Unilateral olfactory functional deprivation model in the left peripheral nostrils by electric cautery injury:olfactory bulb neurogenesis and transformation

作　　者：曾建军[1,2] 贺旭[1] 严小新[1] 罗学港[1] 潘爱华[1] 

机构地区：[1]中南大学湘雅医学院人体解剖学与神经生物学系,湖南省长沙市410013 [2]湖南怀化医学高等专科学校人体解剖学教研室,湖南省怀化市418000

出　　处：《中国组织工程研究》2014年第2期231-238,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81171060)~~

摘　　要：背景:近年来嗅球的神经发生、转化以及成熟已成为研究热点,并且嗅觉经验和神经活动能影响嗅球神经发生,但是到目前为止关于嗅觉功能活动是否影响豚鼠嗅球的神经发生尚无报道。目的:观察单侧嗅觉功能剥夺对幼年豚鼠两侧嗅球微管聚合蛋白、维生素D依赖性钙结合蛋白和小白蛋白表达的影响。方法:将24只幼年豚鼠随机分成2组,通过电烙铁损伤左侧外周鼻孔区建立嗅觉功能剥夺模型,分别于建立嗅觉功能剥夺模型后3,6周灌注取材,应用免疫组织化学技术观察不同时间点豚鼠自身两侧嗅球微管聚合蛋白、小白蛋白和维生素D依赖性钙结合蛋白的表达情况。结果与结论:造模后3周组豚鼠嗅觉未剥夺侧嗅球表达的微管聚合蛋白、小白蛋白和维生素D依赖性钙结合蛋白数目明显高于嗅觉剥夺侧(P<0.05);造模后6周组豚鼠嗅觉未剥夺侧嗅球表达的微管聚合蛋白、小白蛋白和维生素D依赖性钙结合蛋白数目明显高于嗅觉剥夺侧(P<0.05)。提示豚鼠嗅觉功能活动影响神经发生和转化。BACKGROUND： Olfactory bulb neurogenesis, transformation and maturation have been considered the hot topic. Olfactory bulb experience and nervous activity can influence olfactory bulb neurogenesis. However, no study reports that olfactory bulb functions can affect olfactory bulb neurogenesis in guinea pigs. OBJECTIVE： To investigate the effect of unilateral olfactory functional deprivation on doublecortin, calbindin and parvalbumin expression in olfactory bulb of juvenile guinea pigs. METHODS： Totally 24 guinea pigs were randomly divided into two groups, which were killed after establishing olfactory functional deprivation model through electric cautery injury at the left peripheral nostrils. At 3 and 6 weeks after modeling, the specimens were harvested. The expression change of doublecortin, calbindin and parvalbumin in two sides＇ olfactory bulb of juvenile guinea pigs was detected by immunohistochemistry. RESULTS AND CONCLUSION： The number of doublecortin, calbindin and parvalbumin positive cells in olfactory bulb at the un-deprived side was significantly higher than that at the deprived side at 3 and 6 weeks （P 〈 0.05）. This finding indicates that olfactory neural activities can affect neurogenesis and transformation in guinea pigs.

关 键 词：组织构建 组织工程 单侧嗅觉剥夺 嗅球 微管聚合蛋白 维生素D依赖性钙结合蛋白 小白蛋白 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]