链霉亲和素-生物素放大免疫酶法在鼻咽癌诊断中的临床应用  

The Clinical Value of Streptavidin-biotin-amplification Immunoenzymatic Test in the Diagnosis of Nasopharyngeal Carcinoma

作　　者：周珣[1] 吴皖[1] 

机构地区：[1]孝感市中心医院检验科,湖北孝感432000

出　　处：《医学综述》2014年第3期566-567,共2页Medical Recapitulate

摘　　要：目的分析链霉亲和素-生物素放大免疫酶法在鼻咽癌检测当中的临床价值。方法选取2009年3月至2013年1月在孝感市中心医院就诊具有高度鼻咽癌危险的患者326例,分别进行链霉亲和素-生物素放大免疫酶法和常规免疫酶法检验,对链霉亲和素-生物素放大免疫酶法与常规免疫酶法在鼻咽癌患者117例与鼻咽癌高危人群但是无鼻咽癌患者209例的IgA/VCA和IgA/EA的检出率进行观察。结果在鼻咽癌患者中,链霉亲和素-生物素放大免疫酶法的IgA/VCA检出阳性率为99.16%,高于常规免疫酶法的92.31%,IgA/EA检出阳性率为78.63%,高于常规免疫酶法的56.41%(P<0.05)。鼻咽癌高危人群但是无鼻咽癌患者,链霉亲和素-生物素放大免疫酶法的IgA/VCA检出阳性率为24.88%,与常规免疫酶法检出阳性率(23.92%)差别无统计学意义(P>0.05),IgA/EA检出阳性率为3.83%,而常规免疫酶法为0。结论链霉亲和素-生物素放大免疫酶法能够较常规免疫酶法更为灵敏地诊断鼻咽癌。Objective To analyze the clinical value of streptavidin-biotin-amplification immunoenzymatic test in the diagnosis of nasopharyngeal carcinoma. Methods A total of 326 patients with high risk of nasopharyngeal carcinoma from Xiaogan Central Hospital during Mar. 2009 and Jan. 2013 were selected for this study, who received streptavidin-biotin-amplification immunoenzymatic test and routine immunoenzymatic test respectively,then the detection rate of IgA/VCA and IgA/EA of streptavidin-biotin-amplification immu- noenzymatic test and routine immunoenzymatic test in nasopharyngeal carcinoma （ 117 cases ） and normal patients （ 209 cases） were observed. Results The detection rate of IgA/VCA of streptavidin-biotin-amplification immunoenzymatic test in nasopharyngeal carcinoma patients was 99.16% ,which was higher than detection rate of routine immunoenzymatic test（92.31% ） ;the detection rate of IgA/EA was 78.63% ,which was higher than detection rate of routine immunoenzymatic test （56.41% ） （ P 〈 0. 05 ）. The detection rate of IgA/ VCA of streptavidin-biotin-amplification immunoenzymatic test in normal patients was 24.88% ,which had no statistical difference（ P 〉 0.05 ）from routine immunoenzymatic test（23.92% ） ;the detection rate of IgA/EA of streptavidin-biotin-amplification immunoenzymatic test was 3.83%, of routine immunoenzymatic test was 0. Conclusion Streptavidin-biotin-amplification immunoenzymatic test can diagnose nasopharyngeal carcinoma more sensitively than routine immunoenzymatic test.

关 键 词：鼻咽癌 EB病毒 链霉亲和素-生物素放大免疫酶法 

分 类 号：R331[医药卫生—人体生理学]