兔骨性关节炎模型关节软骨的弥散张量研究  

Diffusion tensor imaging study of rabbit osteoarthritis model

作　　者：侯进[1] 梅颖洁[1] 代月黎[1] 许乙凯[1] 

机构地区：[1]南方医院影像中心、南方医科大学,广州510515

出　　处：《中国比较医学杂志》2014年第1期1-4,I0005,共5页Chinese Journal of Comparative Medicine

摘　　要：目的探讨弥散张量成像在膝骨性关节炎的应用价值。方法选用成年新西兰大白兔24只,随机分成为A、B、C、D四组,将A、B、C组兔左膝关节注射木瓜蛋白酶水溶液,建立早、中、晚期关节软骨退变动物模型,D组作为对照组。行膝关节常规扫描及弥散张量成像(DTI)扫描,扫描图像经后处理产生彩色图并与相应解剖图像叠加融合,分别测量关节软骨FA和ADC值。扫描完成后将动物处死,取膝关节软骨采用HE染色与阿尔新兰染色进行对照。结果不同分级标准正常组及模型组软骨的各向异性及扩散特征存在显著的统计学差异;随着退变程度的加重,FA值减低越明显,早期其ADC值先增高后降低。结论 DTI是一种定量化反应兔骨性关节炎严重程度的技术。Objective To explore rabbit osteoarthritis model and evaluate the cartilage degeneration of rabbits by DTI. Methods 24 New Zealand adult rabbits were divided into A, B, C, D groups randomly, and establish articular cartilage degeneration animal model in early middle and later periods, D group as the control group. FA and ADC values of articular cartilage were measured. Observe knee joint cartilage through histological HE staining and Alcian blue staining. Results The cartilage differences of anisotropyand diffusion characteristic between cases and controls were statistically significantly. The FA values gradually decreased while the ADC values increased at early stage then decreased. Conclusions DTI can quantitative analysis rabbit osteoarthritis cartilage degeneration.

关 键 词：弥散张量成像 表观弥散系数 骨性关节炎 

分 类 号：R445.2[医药卫生—影像医学与核医学] R332[医药卫生—诊断学]