甲基泼尼松龙对急性期脊髓损伤bcl-2等凋亡基因的影响  

Effects of methylprednisolone on the neurocyte apoptotic genes such as bcl-2 following acute spinal cord injury

作　　者：郑力恒[1,2,3] 林宏生[3,4] 张国威[3,4] 李锦聪[2] 谢林[1,2] 张大卫[3,4] 

机构地区：[1]澳门仁伯爵综合医院,澳门999078 [2]澳门医学科技研究协会 [3]暨南大学骨科疾病研究所,广州510632 [4]暨南大学附属第一医院骨一区

出　　处：《中国骨与关节杂志》2014年第1期58-62,共5页Chinese Journal of Bone and Joint

基　　金：澳门科学技术发展基金(026/2010/A);广东省科技计划项目(2010-170-1)

摘　　要：目的观察甲基泼尼松龙(methylprednisolone,MP)干预对急性期脊髓损伤凋亡相关基因bcl-2、bax、cytc和caspass-9相对表达量的影响,并探讨该药物促进脊髓功能修复的机制。方法实验动物随机分为假手术组(S组)、单纯损伤组(C组)与MP治疗组(T组)各36只,采用改良Allen's法制作急性脊髓损伤模型,在损伤后8 h、24h、3天、7天、14天和28天随机取各组6只动物处死,实时荧光定量PCR测量标本的△Ct值并进行分析。结果T组bcl-2基因△Ct值均小于C组,差异有统计学意义(P<0.05);bax基因在T组术后3天、14天和28天△Ct值大于C组,差异有统计学意义(P<0.05),T组8 h、24 h和7天三个时间点△Ct值与C组,差异无统计学意义(P>0.05);T组cytc基因△Ct值大于C组,差异有统计学意义(P<0.05);T组caspass-9基因△Ct值大于C组,差异有统计学意义(P<0.05)。结论甲强龙可以通过bcl-、cytc及caspass-9的表达起到保护受伤节段脊髓的作用。Objectives To observe the effects of methylprednisolone （ MP ） on the neurocyte apoptotic genes such as bcl-2, bax, cytc caspass-9 following acute spinal cord injury （ SCI ） and investigate the mechanisms of spinal cord recovery. Methods All experimental rabbits were divided randomly into 3 groups： sham group （ group S, 36 rabbits ）, control group （ group C, 36 rabbits ） and MP treatment group （ group T, 36 rabbits ）. The spinal cord injury models were made according to Allen＇s weight-drop method. 6 rabbits were selected randomly and sacrificed at 8 h, 24 h, 3 d, 7 d, 14 d and 28 d after the injury. The RTFQ- PCR test were taken according to their injured spinal sections, and the results of ACt were compared and analyzed. Results The results of ACt of gene bcl-2 in group T was lower than in group C, which is statistically significant （ P〈0.05 ）. At 3 d, 14 d and 28 d after the injury, the results of ACt of gene bax was higher in group C than in group T, which is statistically significant （ P〈0.05 ）. No significant differences were found between group C and T at 8 h, 24 h and 28 d （ P〉0.05 ）. Group T had better expressions in the ACt of gene cytc and caspass-9 than group C at all time points, which is statistically significant （ P〈0.05 ）. Conclusions MP plays a protective role in treating the acute spinal cord injury by affecting the expressions of the bcl-2, cytc and caspass-9 gene.

关 键 词：脊髓损伤 凋亡调节蛋白质类 功能恢复 甲基泼尼松龙 

分 类 号：R651.2[医药卫生—外科学]