CD74基因调控CXCR4抑制肾癌786-O细胞的侵袭作用  

CD74inhibits 786-O cell invasion in renal cancer by modulating CXCR4expression

作　　者：纪世琪[1] 赵玉千[1] 韩志兴[1] 张海建[1] 程文龙[1] 

机构地区：[1]首都医科大学附属北京地坛医院,北京100015

出　　处：《现代泌尿外科杂志》2014年第2期111-113,共3页Journal of Modern Urology

基　　金：2013年首都医科大学基础临床科研合作课题(No.1300171706)

摘　　要：目的探讨抑制人CD74基因后影响肾癌786-O细胞侵袭能力的作用机制。方法通过慢病毒lentivirus-CD74转染肾癌786-O细胞后,MTT法观察细胞活力变化,Transwell实验观察细胞侵袭能力的变化,应用Westernblot技术检测CD74、CXCR4蛋白的变化情况。结果抑制CD74蛋白表达水平后肾癌786-O细胞活力降低,侵袭能力降低,同时伴有CXCR4蛋白的表达降低。结论通过慢病毒转染抑制CD74的表达可以抑制肾癌细胞的侵袭能力,CD74基因可能是通过调控CXCR4蛋白的变化影响细胞侵袭能力的。Objective To study Methods Lentivirus-CD74 was used to the effect and mechanism of CD74 in inhibiting 786-0 cell invasion in renal cancer transfect renal cancer 786-0 cells,cell vitality was observed with MTT assay,cell inva- sion was observed with transwell assay, and the expressions of CD74 and CXCR4 were detected with Westernblot. Results The transfected lentivirus-CD74 786-0 ceils induced decreased ceil proliferation and invasion,and at the same time, the expres- sion of CXCR4 protein reduced. Oonclusions Transfecting CD74 with lentivirus can inhibit the cell invasion of renal cancer, indicating that CD74 may affect the cell invasion by regulating CXCR4 expression.

关 键 词：肾癌 CD74 CXCR4 侵袭 

分 类 号：Q737.1[生物学—分子生物学]