脉冲Nd-YAG激光控制糜烂型扁平苔藓患者白色念珠菌检出率及分离株毒力的临床研究  

Clinical study of pulsed Nd:YAG laser in the control of Candida albicans prevalence and toxiticy of isolated strain in erosive OLP patients

作　　者：邱宏亮[1] 

机构地区：[1]胜利油田中心医院口腔科,东营257034

出　　处：《实用口腔医学杂志》2014年第1期85-88,共4页Journal of Practical Stomatology

基　　金：东营市科技发展计划(编号:[2009]101-27)

摘　　要：目的:研究脉冲Nd-YAG激光在控制糜烂型口腔扁平苔藓(oral lichen planus,OLP)患者白色念珠菌(Candida albicans,CA)检出率的作用,及对CA分离株毒力的控制。方法:将200例糜烂型OLP患者分为激光组(100例)和碳酸氢钠组(100例)。治疗前和治疗10 d后分别采用浓缩含漱液沙堡平皿培养法,结合芽管试验、厚膜孢子形成试验及温度试验常规培养等方法分离、鉴定各组中念珠菌菌种。将分离、纯化的CA,接种于血琼脂平板上,厌氧培养,检测其溶血活性。结果:治疗前2组CA检出率及菌落数差异无统计学意义(P>0.05)。采用2种不同方法治疗10 d后,CA检出率显著降低(P<0.05),激光组较碳酸氢钠组减少更为显著(P<0.01);用药前所有CA在接种于血琼脂平板培养48 h后均产生了溶血,治疗后CA分离株溶血活性均显著降低(P<0.05),激光组较碳酸氢钠组降低更为显著(P<0.01)。结论:激光能有效控制糜烂型OLP患者CA检出率,并能抑制CA毒力。Objective： To study the effects of pulsed Nd：YAG laser（PNYL） in the control of Candida albicans （CA） prevalence and toxitiey of isolated strain in patients with erosive oral lichen planus （OLP）. Methods： 200 cases of erosive OLP were divided into PNYL group（ n = 100） and sodium bicarbonate（SB） group（ n = 100）. Before and 10 days after treatment, concentrated rinse culture was used with Sabourand plate and combined with the germ tube test, ehlamydospore formation test and temperature test to isolate and identify the type of candida. The isolated and purified CA was inoculated on blood agar, cultured in anaerobic environment, and hemo- lytic activity was detected. Results： Before treatment, there was no statistical significance of the prevalence of CA and colony forming unit（CFU） between the two groups（ P 〉 0.05 ）. After treatment the prevalence of CA was significantly decreased in the 2 groups（ P 〈 0.05）, in PNYL group the effect was more significant than in SB group（P 〈0. 01 ）. Before treatment, hemolysis was seen in all in blood agar plate culture of CA after 48 hours, the hemolysis activity was significantly decreased after treatment（ P 〈 0.05 ）, the effect was more significant in PNYL group than in SB group（ P 〈 0.01 ）. Conclusion： PNYL can control the prevalence of CA in erosive OLP patients and inhibit the toxicity of CA.

关 键 词：口腔扁平苔藓 白色念珠菌 碳酸氢钠 ND-YAG激光 溶血活性 

分 类 号：R758.65[医药卫生—皮肤病学与性病学]