利塞膦酸钠联合脉冲电磁场治疗去势大鼠骨质疏松的实验研究  

Experimental study of risedronate combined with pulsed electromagnetic fields for the treatment of osteoporosis in ovariectomized rats

作　　者：杨华 吴家昌 韦葛堇 农小连 卢其腾 韦诚明 赵秋芬[2] 

机构地区：[1]解放军第三0三医院骨科,南宁530021 [2]广西医科大学

出　　处：《中国骨质疏松杂志》2014年第1期67-72,共6页Chinese Journal of Osteoporosis

基　　金：广西科学研究与技术开发计划课题基金资助(桂科攻10124001-64);广西科学自然基金项目(0991287)

摘　　要：目的观察利塞膦酸钠联合脉冲电磁场对去势大鼠骨质疏松的疗效。方法将32只雌性SD大鼠(250±20)g随机分成4组:正常生长组(Sham)、去卵巢模型组(OVX)、利塞膦酸钠治疗组(RIS)和利塞膦酸钠联合骨质疏松治疗仪治疗组(RIS+PEMFs),每组8只。后三组大鼠行双侧卵巢切除术。去势后12 w RIS组给予0.1 mg·kg-1·d-1利塞膦酸钠灌胃治疗,RIS+PEMFs组给予0.1 mg·kg-1·d-1利塞膦酸钠灌胃和1h·d-1脉冲电磁场治疗,Sham、OVX组每天给予等量生理盐水灌胃。去势后12 w、灌胃治疗4 w、8 w、12 w后四组大鼠均用双能X线吸收法骨密度仪测量全身骨密度(BMD),治疗12 w后进行血生化检测、骨CT值测量和骨组织形态学观察。结果去势后12w后三组BMD与Sham组比较均显著降低(P<0.01),大鼠骨质疏松模型确立;灌胃治疗12 w后,RIS、RIS+PEMFs两组较OVX组BMD(P<0.01)、骨CT值(P<0.01)均较高,且RIS+PEMFs组BMD、骨CT值均比RIS组高(P<0.01);RIS组血清Ca、ALP浓度均较RIS+PEMFs、Sham组高(P<0.05),且RIS+PEMFs组血清ALP浓度较Sham组相比差异无统计学意义(P>0.05);股骨颈病理形态学观察中RIS+PEMFs组的骨小梁形态较完整,较接近Sham组水平。结论去势后12 w可建立大鼠骨质疏松模型;利塞膦酸钠对去势所致骨质疏松大鼠有治疗作用,其与脉冲电磁场联合的治疗效果更为显著。Objective To observe the clinical efficacy of risedronate combined with pulsed electromagnetic fields （ PEMFs） for the treatment of osteoporosis in ovariectomized rats.Methods Thirty-two female SD rats were randomly divided into 4 groups：sham group （Sham）, OVX group （OVX）, risedronate group （RIS）, and risedronate combined with PEMFs group （RIS＋PEMFs）. Each group had 8 rats.Rats in the last 3 groups received the operation of bilateral ovariectomy.At the 12 th week after the operation, rats in RIS group were given a gavage of 0.1mg kg-1 d-1 risedronate, and rats in RIS ＋PEMFs group were received a gavage of 0.1mg kg-1 d-1 risedronate and 1h d-1 pulsed electromagnetic field therapy.Rats in Sham group and OVX group were given a gavage of same volume of normal saline.Bone mineral density （ BMD） of the total body was detected using dual energy X-ray absorptiometry at the 12thweek after ovariectomy and the 4th week, the 8th week, and the 12thweek after the gavage.After 12-week treatment, the bone biochemical detection, bone CT detection, and the histomorphological observation of bone tissues were performed.Results At the 12 th week after ovariectomy, BMD in the last 3 group was significantly lower than that in Sham group （P＆lt;0.01）, indicating the establishment of rat osteoporosis model.After 12-week gavage, BMD in RIS group and RIS＋PEMFs group and the values of bone CT were higher than those in OVX group （P＆lt;0.01）, and the values in RIS＋PEMFs group were also＆amp;nbsp;higher than those in RIS group （P＆lt;0.01）.The serum concentrations of Ca and ALP in RIS group were higher than those in Sham group and RIS＋PEMFs group （P＆lt;0.05）, while no significant difference of serum ALP between RIS＋PEMFs group and Sham group was observed （ P＆gt;0.05 ） .The pathological morphology observation of the femoral neck showed that the bone trabecula in RIS＋PEMFs group was intact, closed to the level in Sham group.Conclusion The rat osteoporosis model can be establish

关 键 词：去势 利塞膦酸钠 骨质疏松 脉冲电磁场 

分 类 号：R68[医药卫生—骨科学]