瓯江彩鲤早期发育中两种脂酶活性及间甲酚对其活性的影响  

Activities of two lipases and effects of m- cresol on them during early development of Cyprinus carpio var. color

作　　者：朱俊华[1] 姚俊杰[1] 冯亚楠[1] 邹芳芳[1] 莫巨鼎 

机构地区：[1]贵州大学动物科学学院水产科学系,贵阳550025

出　　处：《淡水渔业》2014年第1期32-35,共4页Freshwater Fisheries

基　　金：国家自然科学基金(31160527);贵州大学研究生创新基金(研农2013018)

摘　　要：采用生化和静态急性毒性实验方法,研究了瓯江彩鲤(Cyprinus carpio var.color)早期发育过程中脂蛋白脂酶和肝脂酶活性变化及间甲酚对它们活性的影响。结果显示,脂酶在瓯江彩鲤成熟卵和受精卵中均有活性,脂蛋白脂酶和肝脂酶的活性在胚胎发育过程中总体上呈上升趋势,原肠胚期酶活性最高;在水温(20±2)℃条件下,经7 mg/L、13 mg/L和19 mg/L的间甲酚暴露,脂酶活性随着间甲酚的浓度升高先升高后下降,19 mg/L浓度组中肝脂酶活性在胚胎期与其他组均有显著差异(P<0.05);与对照组相比,7 mg/L浓度组脂蛋白脂酶活性在胚胎和仔鱼期均有显著差异(P<0.05)。Activities of lipoprotein lipase （LPL） and hepatic lipase （HL） were determined during early development of Cyprinus carpio var.color, and the effects of m-cresol on them were studied through a biochemical and static toxicological methods.The results showed that lipases were present in the mature eggs and fertilized eggs , and activities of lipase was the highest in the gastrula stage; after being exposed to the m-cresol at the concentration of 7 mg/L, 13 mg/L and 19 mg/L at the temperature （20 ＆#177;2） ℃, activity of lipase first rose and then declined with the increase of m -cresol concentration , HL activity of 19 mg/L concentration group in embryonic stage was significant ly different from other groups （ P＆lt;0.05 ）.Compared to control group , LPL activity of 7 mg/L concentration group in embryonic and larval stage showed significant differences （ P＆lt;0.05 ）.

关 键 词：间甲酚 瓯江彩鲤( CYPRINUS carpio var color) 早期发育 脂蛋白脂酶 肝脂酶 

分 类 号：S917.4[农业科学—水产科学]